银狐精子冷冻前后超微结构的研究  

Study on Ultrastructure of Spermatozoon Before and After Freezing in the Silver Fox(Vulpes fulva)

作　　者：孙凤俊[1] 邴印忠[1] 车丽美[2] 胡庆彬[1] 刘春龙[1] 

机构地区：[1]中国科学院黑龙江农业现代化研究所,哈尔滨150040 [2]东北林业大学野生动物资源学院

出　　处：《黑龙江动物繁殖》2001年第2期1-3,共3页Heilongjiang journal of animal reproduction

基　　金：黑龙江省自然科学基金项目

摘　　要：采用按摩法采集银狐精液 ,将鲜精和冷冻解冻精液离心、固定 ,制备电镜样品 ,通过电镜观察 ,研究精子冷冻前后超微结构变化 ,并进一步研究受精机理和精子冷冻的基础理论 。Massage method was used to collect the semen from healthy silver foxes.the fresh semen and frozen-thawn semen were centrifuged and fixed,respectiverly,and the electronic microscopical samples were prepared for observation.Ultrastrural changes of spermatozoon before and after freezing were studied and at the same time,fertiliztion mechanism and basic theory of sperm freezing have been analyzed furtherly.Thus it can promote development of artificial insemination technique in the fox.

关 键 词：狐狸 精子 冷冻 超微结构 银狐 

分 类 号：S865.23[农业科学—野生动物驯养] S814.3[农业科学—畜牧兽医]