多巴胺(DA)和去甲肾上腺素(NE)在文昌鱼神经系统和性腺中的免疫细胞化学定位  

IMMUNOCYTOCHEMICAL LOCALIZATION OF DOPAMINE AND NOREPINEPHRINE IN THE NERVOUS SYSTEM AND GONADS OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI)

作　　者：翁幼竹[1] 方永强[1] 胡晓霞[1] 周晶[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《动物学报》2001年第3期304-309,T001,T002,共8页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目 (No .3 9870 12 0 )&&

摘　　要：用免疫细胞化学ABC法对多巴胺 (DA)和去甲肾上腺素 (NE)在文昌鱼神经系统和性腺中进行定位研究。免疫反应结果显示 :DA和NE免疫阳性神经细胞广泛分布在文昌鱼端脑后部、中脑前部和中部以及脊髓。我们还观察到 ,在文昌鱼漏斗部也有DA免疫阳性神经细胞及其纤维 ;在中脑中部 ,NE免疫阳性神经细胞及其纤维与另一个神经细胞相接触。此外 ,文昌鱼精巢和卵巢中也存在DA和NE免疫阳性物。研究结果表明 ,DA和NE可能像在鱼类中那样 ,参与调节文昌鱼哈氏窝上皮细胞的分泌活动以及性腺发育。It is well documented that dopamine(DA) and norepinephrine (NE) can regulate gonadotropin secretion in fishes. The presence of DA and NE in the brain of mature Amphioxus [ Branchiostoma belcheri Gray] has been demonstrated by high performance liquid chromatography(HPLC), however, whether these two neurotransmitters are involved in the regulation of the secreton of epithelial cell (primitive gonadotropic cell)of Hatschek's pit (homologous to pituitary in vertebrates ) remains unknown. In the present study, the immunocytochemical localization of DA and NE in the nervous system and gonads of Amphioxus was investigated with (avidin biotin complex ABC) method. Amphioxus (18～52 4mm in total length) were collected from the Tong'an Qiongtou sea area near Xiamen). Each animal was divided into three segments, rostal(from tentaculum peristomial to the first pair of gonads), medial(from the first pair of gonads to the anus) and caudal. They were fixed in Bouin's fixative without acetic acid, and embedded in paraplast. Longitudinal or cross sections of 6 μm thickness were cut. Sections with the structure of the brain, neural tube and gonads were identified under a light microscope and selected for immunostaining. Medium and small types of DA and NE immunopositive nerve cells were found in the posterior part of telencephalon, anterior and middle part of midbrain, and the positive substance occurred in the cytoplasm of nerve cells. No immunoreactivity for DA and NE in this study was detected in the posterior of midbrain and hindbrain of Amphioxus. We found for the first time that DA immunopositive nerve cells and fibers also located in the infundibular part of Amphioxus, a region extending from the middle region of midbrain to the Hatschek's pit and corresponding to the fish infundibular which connected hypothalamus with pituitary. Large type of NE immunopositive nerve cells were showed in the posterior part of telencephalon. NE immunopositive nerve cells and fiber connecting with another nerve cells was observed

关 键 词：文昌鱼 多巴胺 去甲肾上腺素 神经系统 性腺 免疫细胞化学 

分 类 号：Q57[生物学—生物化学]