肌球蛋白单克隆抗体标记大鼠心肌坏死的研究  

ACUTE MYOCARDIAL NECROSIS BY AN IODINATED MONOCLONAL ANTIBODY OF CARDIAC MYOSIN HEAVY CHINS IN RAT

作　　者：朱筱娟[1] 袁茂昆[2] 赵建增[1] 李荣文[1] 高凤桐[2] 侯立中[1] 杨同书[1] 

机构地区：[1]吉林大学生物工程研究所 [2]吉林大学第三临床医学院,长春130021

出　　处：《动物学报》2001年第3期351-354,共4页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目 (No .3 93 70 65 5 )&&

摘　　要：Monoclonal antibody (MAb) attained from hybridoma 1A5 was against human cardiac myosin heavy chains (CMHC) and didn’t react with smooth muscle. It was labeled with Na 125 I and injected to the rats suffered from acute myocardial necrosis, which were treated by isoproylarterenol. After injection, the rats were studied from the 4 th to the 48 th hours by relative radioactivity in blood, thyroid gland, stomach, liver, lung, muscle and heart, respectively. The results indicated that 125 I CMHC MAb could specifically reach the target necrotic organ in the 4 th hours (2 03±0 60 ) and remained up to the 48 th hours (2 55±0 49) or longer, at which the 8 th was higher (4 89±0 44). Meanwhile 125 I CMHC MAb showed much higher affinity to necrotic cardiac tissue than the normal ( P <0 001). The same results were should in the blood from 4 th (4 94±0 45) to 48 th (2 70±0 12). Thus 125 I CMHC MAb can be considered as an effective tool for image diagnosis of acute myocardial necrosis.Monoclonal antibody (MAb) attained from hybridoma 1A5 was against human cardiac myosin heavy chains (CMHC) and didn't react with smooth muscle. It was labeled with Na 125 I and injected to the rats suffered from acute myocardial necrosis, which were treated by isoproylarterenol. After injection, the rats were studied from the 4 th to the 48 th hours by relative radioactivity in blood, thyroid gland, stomach, liver, lung, muscle and heart, respectively. The results indicated that 125 I CMHC MAb could specifically reach the target necrotic organ in the 4 th hours (2 03±0 60 ) and remained up to the 48 th hours (2 55±0 49) or longer, at which the 8 th was higher (4 89±0 44). Meanwhile 125 I CMHC MAb showed much higher affinity to necrotic cardiac tissue than the normal ( P <0 001). The same results were should in the blood from 4 th (4 94±0 45) to 48 th (2 70±0 12). Thus 125 I CMHC MAb can be considered as an effective tool for image diagnosis of acute myocardial necrosis.

关 键 词：肌球蛋白 心肌坏死 单克隆抗体 标记 大鼠 

分 类 号：R392.12[医药卫生—免疫学]