雨生红球藻营养细胞的虾青素累积  

ASTAXANTHIN ACCUMULATION IN VEGETATIVE CELL OF HAEMATOCOCCUS PLUVIALIS

作　　者：庄惠如[1] 卢海声[1] 陈必链[1] 陈文列[2] 施巧琴[1] 

机构地区：[1]福建师范大学生物工程学院,福州350007 [2]福建医科大学电子显微镜室,福州350004

出　　处：《水生生物学报》2001年第4期376-380,共5页Acta Hydrobiologica Sinica

基　　金：福建省自然基金资助项目 (C970 2 0 ) ;福建省计委资助项目

摘　　要：接种于缺氮培养基的雨生红球藻。当置于光强为 1 0— 1 2klx ,温度 2 5℃± 1 5℃条件下培养时 ,营养细胞迅速由绿色变成红色。接种 4d时 ,运动细胞占细胞总数的 90 % ,细胞内的虾青素含量 (33 0 pg/cell)占最终色素累积量的 (60 0 pg/cell)的 55%。接种 6d时 ,虾青素含量已占最终累积量的 75%。电镜观察显示 ,红色的运动细胞除细胞质的大部分区域充满色素颗粒外 ,细胞的形态结构与绿色细胞基本一致。实验还表明 ,当接种物置于低光 (0 5—1 0klx)下培养时 ,营养细胞仍有色素沉积 ,但累积缓慢。当只有高光胁迫 (1 0— 1 2klx ,完全培养基培养 )时 ,营养细胞转变成厚壁孢子后 (5d后 ) ,才大量积累色素。When H.pluvialis was inoculated in a nitrogen-deficient medium and exposed to high light intensity condition (10—12 klx), the change of green motile cells to red motile cells was observed in four days. After four days of inoculation, the number of vegetative cells was 90% of total number of cells and the astaxanthin content (33.0 pg/cell) was 55% of the final astaxanthin content (60.0 pg/cell) which was inoculated ten days. After six days, the astaxanthin content was 75% of final astaxanthin content. It was found by electron microscopic observation that the cytoplasm of red motile cells was almost full of pigment granules, but the morphology and structure of cell was identical with green motile cells. The experiments also showed that the astaxanthin accumulation in motile cells would occur too when H.pluvialis was exposured to nitrogen starvation but lower light intensity condition (0.5—1.0 klx). When H.pluvialis was only exposured to high light-stress condition(inoculated in complete medium), the large amount pigment accumulation was only found during the transformation of vegetative cells to akintes.

关 键 词：雨生红球藻 营养细胞 虾青素胁迫条件 超微结构 

分 类 号：Q949.212[生物学—植物学]