BBTV两个株系DNA组分3的克隆及序列分析  

Cloning and Sequencing of DNA Component 3 of BBTV Two Strains

作　　者：何自福[1] 肖火根[1] 李华平[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室

出　　处：《农业生物技术学报》2001年第2期145-148,共4页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(39870434); 广东省自然科学基金资助项目(980126); 高等学校博士点基金(950503)

摘　　要：通过常规的基因克隆技术，对广东ＢＢＴＶ两个株系 （ＮＳＰ株系和ＮＳ株系）的代表分离物（广州天河分离物和高州分离物）的ＤＮ Ａ组分３进行了克隆和序列分析。结果表明：该组分的核苷酸全序列、ＯＲＦ及其推导编码的氨基酸序列在两个代表分离物间的变异率分别为４．２％、２．５％和５．９％，两者差异显著，从而进一步支持了广东ＢＢＴＶ存在两个株系的结论。此外，这两个株系的ＤＮＡ组分３、ＯＲＦ及其推导编码的氨基酸序列与肖火根等报道的中国（广东）分离物、澳大利亚分离物分别进行比较，结果表明：在这两个株系中，ＮＳ株系与中国（广东）分离物的亲缘关系最密切，可以认为是属同一株系（ＮＳ株系）；而这两个株系与澳大利亚分离物的亲缘关系均较远。By regular gene cloning techniques, DNA component 3 of the two isolates (Guangzhou-Tianhe isolate and Gaozhou isolate) representing respectively BBTV NSP strain and NS strain were cloned and sequenced. The results showed that the variance ratios of the full sequences of the component, the ORF nucleotide sequences, and its encoding amino acid sequences between the two isolates were 4.2%, 2.5% and 5.9% respectively Those experimental results showed that the differences of the components between the two representative isolates were significant. In addition, respectively comparing DNA component 3, the ORF nucleotide sequences and its encoding amino acid sequences of the representative isolate of the two strains with those of Xiao's Chinese (Guangdong) isolate and the Australia isolate, the results showed that the Chinese (Guangdong) isolate was closely related to the representative isolate of the NS strain, and should be grouped into the NS strain, that the two st fains were distant with the Australia isolate.

关 键 词：香蕉束顶病毒 株系 基因克隆 序列分析 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]