根癌农杆菌转化栝楼及植株再生  

Genetic Transformation of Trichosanthes kirilowii Maxim. by Ti Plasmid and Formation of Plantlets

作　　者：雷和田[1] 宋经元[1] 祁建军[1] 张荫麟[1] 杨峻山[1] 

机构地区：[1]中国医学科学院协和医科大学药用植物研究所,北京100094

出　　处：《植物学通报》2001年第3期363-366,共4页Chinese Bulletin of Botany

摘　　要：用根癌农杆菌 (Agrobacteriumtumefaciens)菌株C58感染栝楼 (TrichosantheskirilowiiMaxim .)无菌苗诱导冠瘿瘤 ,获得其冠瘿组织 ;栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长 ,纸电泳检测结果表明其合成了冠瘿碱 ,表明Ti质粒转化成功。栝楼冠瘿组织在MS培养基上形成完整植株 ,移栽后良好生长。Crown galls were induced by direct infection of sterilized seedlings of Trichosanthes kirilowii Maxim. with Agrobacterium tumefaciens C58. Transformation of Ti in C58 was inspected by high_pressure_paper electrophoresis; the detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully. The crown tissues produced plantlets on MS medium and the plantlets were successfully transplanted and grew well.

关 键 词：栝楼 Ti质粒转化 植株再生 根癌农杆菌 

分 类 号：Q93-3[生物学—微生物学]