葛根素对全脑缺血再灌流后脑组织诱导型一氧化氮合酶影响的实验观察  

Observation of the puerarin's effect on cerebral inducible nitric oxide synthase during global cerebral ischemia and reperfusion

作　　者：高鹏[1] 夏成青[2] 王佩燕[1] 刘禹赓[1] 梅雪[1] 

机构地区：[1]首都医科大学附属北京红十字朝阳医院急诊科,100020 [2]首都医科大学附属北京红十字朝阳医院病理科

出　　处：《中华急诊医学杂志》2001年第2期85-86,共2页Chinese Journal of Emergency Medicine

摘　　要：目的　观察葛根素对全脑缺血再灌流后脑组织诱导型一氧化氮合酶的影响。方法　将实验大鼠随机分为空白对照组、缺血再灌流盐水组及葛根素组 ,采用Pulsinelli4血管阻塞法造成全脑缺血再灌流模型 ,用免疫组织化学染色法行大鼠脑组织的iNOS测定。结果　随缺血再灌流时间的延长 ,盐水组iNOS逐渐降低 ,较空白对照组差异明显 (P <0 0 5 ) ,且再灌流时间越长 ,此差异越显著 (P<0 0 1) ;而葛根素组iNOS逐渐增高 ,缺血再灌流 2 4h时 ,葛根素组较盐水组iNOS明显增高 (P <0 0 5 ) ,接近空白对照组。结论　全脑缺血再灌流后 ,脑组织iNOS系统有明显损伤 ,葛根素可减轻这种损伤。Objective To obseve the effect of puerarin on cerebral inducible nitric oxide synthase(iNOS) during global cerebral ischemia and reperfusion.Methods The rats were randomly divided into three groups:control group(only exposed anatomically without cerebral ischemia and reperfusion);normal saline(NS) group(global cerebral ischemia and reperfusion with administration of NS through abdomen);puerarin group(global cerebral ischemia and reperfusion with administration of puerarin through abdomen).Global cerebral ischemia and reperfusion was induced by means of Pulsinellis four blood vessel occlusion model.iNOS in rat brain was measured with immuno histochemical staining method.Results As the time of reperfusion lasted,the level of iNOS in NS group declined markedly(P<0 05),and the longer the period of reperfusion,the more the difference(P<0 01)between the NS group and the control group;whereas iNOS in puerarin group increased gradually, the level of iNOS of puerarin group was much higher than that of NS group at 24h reperfusion(P<0 05).Conclusion Cerebral iNOS system can be markedly damaged after global cerebral ischemia and reperfusion,puerarin can lessen this damage.

关 键 词：葛根素 全脑缺血 再灌流损伤 脑组织 诱导型一氧化氮合酶 

分 类 号：R285.6[医药卫生—中药学]