体内外诱导CD34^+细胞生成血管内皮细胞的方法及其组织工程学运用  

Induction of CD34^+ Cell into Endothelial Cell and Application in Tissue Engineering

作　　者：谭强[1] 沙慧芳[2] 江晓丰[2] 史振余[2] 顾伟勇[2] 周允中[1] 

机构地区：[1]上海市胸科医院胸外科,上海200030 [2]上海市胸科医院肿瘤研究室,上海200030

出　　处：《复旦学报（医学版）》2001年第3期191-194,T001,共5页Fudan University Journal of Medical Sciences

摘　　要：目的　本实验设计从骨髓中提取CD34 + 细胞作为血管内皮细胞干细胞 ,经诱导生成血管内皮细胞应用于组织工程学器官重建术。方法　利用免疫磁珠法从骨髓中提纯的CD34 + 细胞在体外经血管内皮细胞生长因子 (VEGF)、成纤维细胞生长因子 (bFGF)诱导 ;在动物实验中 ,CD34 + 细胞经Dil标记后于Matrigel混合涂于裸鼠背部皮肤缺损处 ,表面覆盖人胸膜组织。结果　细胞经诱导可转化生成血管内皮细胞 ,Ⅷ∶Ag、CD31+ 染色阳性 ,并可在重组基底膜 (Matrigel)中形成三维毛细血管网状结构。体内实验结果可见CD34 + 细胞参与并加速胸膜下毛细血管网形成 ,短时期内重建血供系统确保胸膜组织存活。对照组 (单纯覆盖Matrigel与胸膜 )胸膜缺血坏死。结论　可利用CD34 + 细胞替代毛细血管内皮细胞重建正常组织毛细血管网 ,与器官实质细胞共同组成复合组织 ,运用于组织工程化器官重建术 。Purpose To apply CD34 + cell as endothelial progenitor cell to tissue engineering organ reconstruction. Methods First we isolated CD34 + cells from bone marrow by magnetic bead selection,then induced them in the presence of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(bFGF).Further in the animal experiment,we made a skin loss on the back of nude mice,applied matirgel mixed with human CD34 + cells onto the trauma,then we implanted pleura on the reconstructive connective tissue. Results CD34 + cells were able to be induced into endothelial cells,which are CD34 +,CD31 + and stain for Ⅷ∶Ag,and formed capillary structure in the Matrigel.We also found them accelerated the reconstruction of the capillary network under the pleura in vivo ,while the control group with poor capillary density and couldn't maintain the living of epithelial tissue. Conclusions With the advantage of CD34 + we can get tissue engineering prosthesis with the ability of forming capillary network in the shortest time to maintain the living of parenchymal cells.

关 键 词：CD34^34+细胞 血管内皮细胞 再血管化 组织工程学 

分 类 号：Q813[生物学—生物工程]