TGF-β1对大鼠系膜细胞增殖和纤溶酶原激活物及其抑制剂表达的影响  

Effects of TGF-β1 on Cells Growth and Expression of Plasminogen Activators and its Inhibitor on Cultured Rat Mesangial Cells

作　　者：林文生[1] 张农[1] 张颂文[2] 顾建新[2] 郭慕依[1] 

机构地区：[1]复旦大学基础医学院病理学教研室,上海200032 [2]复旦大学基础医学院基因研究中心,上海200032

出　　处：《复旦学报（医学版）》2001年第3期213-215,共3页Fudan University Journal of Medical Sciences

基　　金：上海市科技发展基金 (98QB14 0 0 7) ;卫生部优秀青年科技人才专项基金;上海市教委重点学科基金 (B990 80 2 )资助课题

摘　　要：目的　通过对大鼠系膜细胞转染TGF β1基因和反义TGF β1基因 ,观察该细胞增殖和纤溶酶原激活物及其抑制剂表达的影响。方法　采用MTT、细胞计数和流式细胞仪检测细胞增殖 ;Northernblot和Westernblot法检测其纤溶酶原激活物抑制剂 1(PAI 1)的表达 ;酶谱分析法检测其纤溶酶原激活物 (tPA)活性。结果　过表达TGF β1的系膜细胞生长受抑制 ,其PAI 1mRNA和蛋白表达增强 ,而tPA酶活性则降低 ;而低表达TGF β1的系膜细胞增殖加速 ,其PAI 1mRNA和蛋白表达降低 ,tPA酶活性增强。结论　TGF β1可通过对大鼠系膜细胞增殖、纤溶酶原激活物及其抑制因子表达的影响 。Purpose: To study the effects of transforming growth factor-β1 (TGF-β1) on cells growth and expression of plasminogen activators and its inhibitor on cultured rat mesangial cells transfected with TGF-β1 gene and antisense TGF-β1 gene. Methods: MTT array, cell counting and FACS analysis for examining mesangial cells proliferation. Northern blot and Western blot analysis for detecting the levels of plasminogen activator inhibitor-1 (PAI-1) mRNA and protein, respectively. Zymography analysis for determining the activity of tissue type plasminogen activators (tPA). Results: Overexpression of endogenous TGF-β1 inhibited mesangial cell growth, increased the level of PAI-1 mRNA and protein, and decreased the astivity of tPA. Conversely, lower-expression of TGF-β1 stimulated mesangial cells proliferation, decreased the lerel of PAI-1 mRNA and protein, and increased the activity of tPA. Conclusions: TGF-β1 as a potent inhibitor of mesangial cells growth and a regulator of tPA/PAI-1 may play an important role in the development of glomerular fibrosis.

关 键 词：肾小球系膜细胞 转化生长因子-Β1 细胞增殖 纤溶酶原激活物抑制剂-1 纤溶酶原激活性 大鼠 

分 类 号：R692.6[医药卫生—泌尿科学] Q754[医药卫生—外科学]