红豆杉细胞聚集体悬浮培养生产紫杉醇  

Initiation, Growth and Paclitaxol Production of Taxus chinensis Cell Aggregate Suspension Cultures

作　　者：刘华文[1] 张长河[1] 刘幸福[1] 吴元喜[1] 

机构地区：[1]华中科技大学生命科学与技术学院

出　　处：《华中科技大学学报（自然科学版）》2001年第A01期44-47,共4页Journal of Huazhong University of Science and Technology(Natural Science Edition)

基　　金：国家"九五"重点科技攻关项目 (96 C0 2 0 3 0 1) ;国家高技术研究发展计划资助项目 (10 2 12 0 6 0 1)

摘　　要：建立了中国红豆杉 (Taxuschinensis)细胞聚集体两步法悬浮培养生产紫杉醇的培养体系 .红豆杉细胞于含LH 1g/L ,NAA 0 .1mg/L ,2 ,4 D 0 .5mg/L及 6 BA 0 .8mg/L的细胞聚集体形成的培养基中生长并形成聚集体 ,10d后转入生产培养 ,较大细胞聚集体的生物量比和紫杉醇产量分别较对照组提高了 5 3 .7%和82 .1% ;提高细胞聚集体的生物量比能显著增加紫杉醇产量 .四种基本培养基中B5最适合诱导细胞聚集体的形成 .生长培养 12d后细胞悬浮培养物按 1∶1(体积比 )直接转入生产培养对细胞生长、细胞聚集体和紫杉醇形成最有利 .在 10 0A two step system of Taxus chinensis cell aggregate suspension cultures for enhancing paclitaxel production is presented. After the cells are cultured in the growth medium containing LH1?g/L, NAA 0.1?mg/L, 2,4 D 0.5?mg/L, 6 BA 0.8?mg/L for 10 days, cell aggregates are developed. The cell aggregates transferred to the production medium containing 100?μmol/L methyl jasmonate give a 53.7?% higher cell aggregate biomass ratio and a 82.1?% higher paclitaxel level than those of the control, respectively. That achieving more cell aggregates can result in a higher paclitaxel concentration. B 5 is the best one among the four basic media used for cell growth and cell aggregate formation increasing paclitaxel production. After cell aggregate suspension cultures are incubated in the growth medium for 12 days, they are inoculated into the production medium at 1∶1 (by vol.) leading a two fold paclitaxel content as compared to the control.

关 键 词：红豆杉 组织培养 紫杉醇 细胞聚集体 两步法培养 生物制药 

分 类 号：TQ464[化学工程—制药化工]