晚期丝虫病患者血清特异性抗体及抗体亚型的分析  

IMMUNOLOGY ANALYSIS ON SPECIFIC ANTIFILARIAL ANTIBODY AND SUBCLASS OF CHRONIC FILARIASIS CASES

作　　者：王世海 刘涛 陈兆义 郑惠君 

机构地区：[1]贵州省寄生虫病研究所,贵州贵阳550004

出　　处：《中国寄生虫病防治杂志》2001年第2期107-108,共2页Chinese Journal of Parasitic Disease Control

摘　　要：目的　评价晚期丝虫病患者血清抗丝虫抗体及抗体亚型的免疫学特性。　方法　用马来丝虫成虫和微丝蚴抗原 ,以 EL ISA法检测 12 0份血清标本抗丝虫特异性抗体和抗体亚型 ,FPT方法进行丝虫皮内试验。　结果　病原学检查12 0份受试者均为非微丝蚴血症。80例晚期丝虫病患者 FPT试验阳性率为 95 % (76 / 80 ) ,晚期丝虫病患者血清抗微丝蚴和成虫特异性抗体阳性率分别为 82 .5 % (6 6 / 80 )和 80 % (6 4/ 80 )。流行区对照抗体阳性率为 10 % (2 / 2 0 ) ,非流行区正常人则均为阴性。晚期丝虫病患者血清抗体亚型以 Ig G2为主 ,阳性率 92 .5 % ,其它亚型分别为 Ig G11.2 5 %、Ig G32 2 .5 %、Ig G46 .2 5 %。　结论　晚期丝虫病患者血清中存在抗丝虫特异性抗体 ,抗体亚型主要是 Ig G2 。Objective To evaluate immunological responses of chronic filariasis cases(chyluria, hydrocoele, elephantiasis). Methods The serum specific antifilarial antibodies and antibody subcalss level of 120 sera samples from chronic filariasis cases and controls were measured by ELISA with PBS extract of B. malayi microfilariae (Mf) and adult worm, skin reaction was also tested with FPT in all human subjects. Results 95%(76/80) positive reaction of FPT test were observed, and 82.5%(66/80) and 80%(64/80) subjects kept developed antibodies to Mf and adult worm extracts respectively in chronic filariasis cases. However, antibody positive rate was 10%(2/20) in sera from endemic area controls, but was 0 in sera from unendemic area control. The positive rate of major antibodies subclass IgG2 was 92.5%. The positive rate of IgG1, IgG3 and IgG4 subcalss was 1.25%, 22.5% and 6.25%, respectively.Conclusion IgG2 was major subclass of specific anti-filarial antibodies in sera from chronic filariasis cases.

关 键 词：丝虫病 晚期 抗体 抗体亚型 

分 类 号：R532.15[医药卫生—内科学]