流式细胞术测定感染旋毛虫小鼠免疫功能的研究  

STUDY ON THE IMMUNE FUNCTION DETECTION OF MICE INFECTED WITH TRICHINELLA SPIRALIA BY FLOW CYTOMETRY

作　　者：申丽洁[1] 罗志勇[2] 朱声华[3] 

机构地区：[1]大理医学院寄生虫学教研室,云南大理671000 [2]大理医学院医学系 [3]华西医科大学寄生虫学教研室

出　　处：《中国寄生虫病防治杂志》2001年第2期112-113,共2页Chinese Journal of Parasitic Disease Control

摘　　要：目的观察感染旋毛虫小鼠免疫功能的动态变化。方法采用流式细胞术分别检测感染旋毛虫后 7、14、2 1、2 8、35 d小鼠外周血 CD4+、CD8+ T淋巴细胞的百分率。结果实验组较正常组 CD4+ T细胞减少 ,CD8+ T细胞增多 ,CD4+ / CD8+细胞比值下降 (P<0 .0 5 ) ,以感染后第 14d最明显 ,直到感染后 35 d仍未见恢复。结论感染旋毛虫的小鼠出现免疫抑制现象 ,感染急性期的免疫抑制程度最高。Objective To observe the dynamic changes of T lymphocyte subsets in mice infected with Trichinella spiralis. Methods The percentage of CD 4+ and CD 8+ T cells were examined by flow cytometry on 7,14,21,28,35 days after mice infected with T. spiralis respectively. Results In experimental group, CD 4+ T cells decreased, CD 8+ T cells increased, the ratio of CD 4+/CD 8+ cells decreased and which was the obvious on the 14th days comparing with normal group (P<0.05). Conclusion The immune function of mice infected with T. spiralis was inhibited, and the degree of immune inhibition was the highest in the acute phase.

关 键 词：流式细胞术 旋毛线虫 T淋巴细胞 免疫抑制 

分 类 号：R383.15[医药卫生—医学寄生虫学]