转基因抗虫棉Bt基因不同剂量的聚合与抗虫性表现  

Investigation of Different Dosages of Inserted Bt Genes and Their Insect-resistance in Transgenic Bt Cotton

作　　者：郭旺珍[1] 孙敬[1] 郭玉芳[1] 张天真[1] 

机构地区：[1]南京农业大学棉花研究所作物遗传与特异种质创新教育部重点实验室,南京210095

出　　处：《Acta Genetica Sinica》2001年第7期668-676,共9页

基　　金：国家"863"高科技研究项目

摘　　要：通过有性杂交手段培育出聚合有不同数目Ｂｔ基因的植株，在不同生育期进行抗虫性测定和Ｂｔ毒蛋白表达的ＥＬＩＳＡ检测，旨在揭示聚合不同数目Ｂｔ基因的植株抗虫性的互作表达机理．聚合有１—４个Ｂｔ基因的植株在整个生育期的抗虫性、毒蛋白表达特性和单价抗虫棉时空表达一致，生育前期抗虫性好，毒蛋白表达量高；生育中、后期抗虫性有所下降，毒蛋白表达量降低。聚合有４个Ｂｔ基因的纯合材料并未因Ｂｔ基因的增加而起到抗性增强的效果，相反还因同源抑制而有所降低．不同来源的Ｂｔ基因处于杂合状态时其抗虫性和Ｂｔ毒蛋白量均得到充分表达。The plants with various dosages of inserted Bt genes were developed by intercrossing among transgenic Bt lines in which Bt genes were inserted in different chromosomes, and commercial non-transgenic Bt varieties. Laboratory bioassay of insect-resistance and Bt toxin protein assays with ELISA were used to detect efficacy to bollworm in different cotton growing stages. As transgenic Bt insect-resistant cotton, temporal difference of resistance existed in plants converging on different dosages of inserted Bt genes, i.e., there was a declining level of efficacy with plant age as the mortality (%) of Helicoverpa and Bt toxin protein expressional level decreased gradually, when analyzed with leaves from the main stem. Our research also revealed that the insect-resistance of converging 4 Bt genes plants was decreased due to homozygous suppression.

关 键 词：转BT基因棉花 聚合 抗虫性 育种利用 剂量 

分 类 号：Q943[生物学—植物学]