人SCF基因5′旁侧-1190～- 853 AT富集区功能研究  

Characterization of the AT Rich Region from -1190 to -853 of 5′ Flanking Sequence of Human Stem Cell Factor Gene

作　　者：聂怡玲[1] 谭文斌[1] 朱敏[1] 罗赛群[1] 郭小珊 成光杰 胡维新[1] 彭兴华[1] 

机构地区：[1]中南大学湘雅医学院分子生物学研究中心,长沙410078

出　　处：《中国生物化学与分子生物学报》2001年第3期315-318,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金 (No .3 970 0 0 5 0 ;3 990 0 15 9)资助项目&&

摘　　要：已有的报告基因和EMSA实验研究表明 ,人干细胞生长因子 (SCF)基因 5′旁侧AT富集区- 1 1 90～ - 853在HeLa和MCF 7细胞中均能增强下游基因转录 ,可能为一个核基质结合区(MAR) ,对人SCF基因的转录发挥调控作用 .为进一步研究该AT富集区的功能 ,将人SCF基因 5′旁侧 - 1 1 90～ - 853AT富集区分别克隆入SV40或CMV启动子前后紧接着CAT报告基因 ,瞬时转染Jurkat,HepG2和 3T3细胞 ,检测CAT报告基因的瞬时表达活性 .结果表明 :人SCF基因 5′旁侧- 1 1 90～ - 853AT富集区在Jurkat和HepG2细胞中 ,对分别由SV40和CMV启动子引导的CAT基因表达均有抑制作用 ;但在 3T3细胞中对SV40启动子的转录活性表现出增强作用 ,对CMV启动子的转录活性无明显影响 .这些结果提示 ,人SCF基因 5′旁侧 - 1 1 90～ - 853AT富集区转录调控具有组织细胞特异性 。It was shown that the AT rich region from -1190 to -853 of 5′ flanking sequence of human stem cell factor (SCF)gene could enhance the transcription in both HeLa and MCF 7 cells by reporter gene and EMSA studies,as it might be a matrix associated region(MAR).The AT rich region from -1 190 to -853 of 5′ flanking sequence of human SCF gene was cloned upstream to SV40 or CMV promoter linked to a CAT reporter gene.Transient transfection experiments were performed to detect the expressional activites of CAT reporter gene in Jurkat,HepG2 and 3T3 cells.The results showed that the AT rich region from -1 190 to -853 of 5′ flanking sequence of the human SCF could significantly inhibit the activities of both promoters in Jurkat and HepG2;while it could improve the activity of SV40 promoter,and had little effect on the activity of CMV promoter in 3T3 cell.The results indicated that the AT rich region from -1190 to -853 of 5′ flanking sequence of the human SCF had a tissue specific regulatory function,it might suppress or improve the transcriptional activities of promoters in different cells.

关 键 词：人干细胞因子基因 调控元件 启动子 报告基因检测 SCF 

分 类 号：Q75[生物学—分子生物学]