羟基丁酸酯和羟基己酸酯共聚物的神经亲和性研究  

STUDIES ON THE NERVE CELL AFFINITY OF POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE)(PHBHH_X)

作　　者：杨吟野[1] 李训虎[1] 龚海鹏[1] 陈国强[1] 赵南明[1] 张秀芳[1] 

机构地区：[1]清华大学生物科学与技术系

出　　处：《生物物理学报》2001年第2期261-266,共6页Acta Biophysica Sinica

基　　金：国家863计划基金资助项目!(No.819-07 -01)

摘　　要：PHA具有良好的力学性能并能完全被生物降解 ,PHBHHX 是短链和长链单体共聚的PHA ,具有硬度和韧性都好的特点。研究PHBHHX 的亲水性、保持蛋白质在材料上有序结构变化的情况及Hela细胞和鼠大脑皮层神经细胞在材料上的生长情况 ,说明PHBHHX 是很有希望的神经修复材料 ,并且通过热分析法 (DSC)得到其熔点 ,使之便于以后的处理加工。PHA is a kind of materials with excellent mechanical properties and complete biodegradability. PHBHHX, long chain and short chain monomer polymerized PHA, has a better tenacity and rigidity. In our experiment, Hela cells and fetal rat cerebral cortex nerve cells were cultured on PHBHHX respectively, and the nerve cell affinity of the materials were evaluated by measuring the water contact angle of the materials and the circular dichroism(CD) of proteins adsorbed on the materials. Results indicate that PHBHHX is one of the promising nerve restoring materials. DSC method was used to determine the melting point of the materials so that it will be convenient to process the materials later.

关 键 词：PHBHHX HELA细胞 神经细胞 蛋白吸附 神经亲和性 可降解材料 PHA 

分 类 号：Q421[生物学—神经生物学] R318.08[生物学—生理学]