新肝炎病毒TTV在各类高危人群中的感染状况和分子流行病学研究  

A CLINICAI AND MOLECULAR EPIDEMIOLOGIC STUDY OF TT VIRUS IN VARIOUS POPULATION

作　　者：郑煜煌[1] 胡维新[2] 黄力[1] 何艳[1] 

机构地区：[1]湖南医科大学附属第二医院肝病研究中心,湖南长沙410011 [2]湖南医科大学分子生物研究中心,湖南长沙410008

出　　处：《中国微生态学杂志》2001年第3期149-152,共4页Chinese Journal of Microecology

摘　　要：目的　观察新肝炎病毒 TTV在各类高危人群中的感染状况、基因分型及其在肝病发生和发展过程中的作用。方法　在日本株 TTV ORF1 保守区设计了两对套式引物 ,采用巢式聚合酶链反应扩增血清 TTV DNA,并对产物进行分子克隆和部分基因序列分析。结果　在非甲 -戊型和非庚型肝炎病人、血清HBs A g阳性的肝炎病人、正常献血员、肝炎肝硬化病人、原发性肝癌病人、静脉内吸毒者和女性与男性性乱者中 ,血清 TTV DNA 阳性率分别为 43.2 % (16 /37)、2 8.8% (15 /5 2 )、9.3% (4/4 3)、5 1.9% (14/2 7)、38.5 % (5 /13)、35 .0 % (14/4 0 )、17.3% (8/4 5 )和 18.8% (3/16 )。其中非甲 -戊型和非庚型肝炎病人、血清HBSAg阳性肝炎病人的 AL T平均为 (472± 2 76 ) u· L- 1 和 (385± 2 18) u· L- 1 ;肝炎肝硬化病人 TTV DNA阳性率显著高于 HBSAg阳性肝炎病人。同时 ,从非甲 -戊型和非庚型肝炎病人、血清 HBSAg阳性肝炎病人、正常献血员、静脉内吸毒者和女性性乱者中 ,分别获得 6份 TTV DNA ORF1 克隆 ,其基因序列与日本株TTV ORF1 部分基因核苷酸序列同源性为 97%～ 99% ,均属于 TTV 1a型。结论　 TTV感染和 AL T升高存在一定的关系 ;我国各类高危人群感染 TTV以 1a型为主 。Objective:To investigate infection an sequence divergence of TT virus in various population,and the role in chronic liver disease development.Methods:A nested polymerase chain reaction (nPCR) assay with primers from TTV ORF 1 genome was used to detect serum TTV DNA in various population,nPCR products were cloned and sequenced.Results:TTV DNA was positive in sixteen of thirty seven patients with non A through non E and non G hepatitis(43 2%),fifteen of fifty two patients with HBsAg positive(28 8%),four of forty three normal blood donors(9 3%),fourteen of twenty seven patients with hepatocirrhosis(51 9%),five of thirteen patients with primary hepatic carcinoma (38 5%),fourteen of forty intravenous drug users(35.0%),eight of forty five woman prostitutes(17 3%),and three of sixteen sexual men(18 8%).Elevated transaminase(ALT)was observed in the patients with non A through non E and non G hepatitis,and with HBsAg positive(472u·L 1 and 385u·L 1 ,respectively).Six gene sequences were compared with known sequence of TTV isolate(N22) reported by Okamoto.The nucleotide homology were 97%～99% and whole belong to Gla gene type.Conclusion:The data indicate that TTV infection correlated with abnormal ALT,which might be a pathogen for non A through non G hepatitis.TTV DNA form various population in China might chiefly be la genotype and there is no correlation between genotype and disease trasmission and development.

关 键 词：肝炎病毒 聚合酶链反应 感染 DNA序列分析 TTV 

分 类 号：R373.21[医药卫生—病原生物学] R512.6[医药卫生—基础医学]