过碘酸盐氧化法制备固相载体除抗非目的蛋白抗体  

REMOVING UNSPECIFIC ANTIBODIES WITH SEPHACRYL GEL COUPLING OF E COLI PROTEIN BY PERIODATE OXIDATION

作　　者：刘相国[1] 杨恭[1] 邱并生[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学通报》2001年第3期73-75,共3页Microbiology China

摘　　要：Sephacryl凝胶经过碘酸盐氧化将产生醛基 ,非目的蛋白通过氨基与醛基反应结合到凝胶上制备固相载体。装柱后 ,抗体经过长时间循环上样 ,抗非目的蛋白的抗体被吸收 ,抗体得到了纯化。用经本法处理过的抗体做免疫印迹 。The total proteins of plain E.coli(DH5 α)were coupled to the Sephacryl S300 oxidized with periodate,and the preliminarily purified antiserum was loaded repeatedly on the column for 18 hours.The unspecific anti\|E.coli antibodies would be absorbed and the antibodies would be purified.It was more specific to do western blot using antibodies treated by this method than using ones without treatment.

关 键 词：Sephacry1凝胶 抗体 免疫印迹 过碘酸盐氧化法 固相载体 抗血清 

分 类 号：Q939.9[生物学—微生物学]