中国棉铃虫单粒包埋核多角体病毒(HaSNPV)HindⅢ-Ⅰ片段的序列分析与基因排列研究  

Genetic Organization of the HindⅢ-Ⅰ Region of the Single Nucleocapsid Nucleopolyhedrovirus of Helicoverpa armigera (HaSNPV)

作　　者：龙钢[1] 陈新文[1] Just M.Vlak 胡志红[1] 

机构地区：[1]中国科学院武汉病毒研究所无脊椎动物病毒学联合开放实验室,湖北武汉430071 [2]Department of Virology,Wageningen University

出　　处：《病毒学报》2001年第2期150-157,共8页Chinese Journal of Virology

基　　金：国家自然科学基金 ( 3 9980 0 0 1;3 9970 0 3 1) ;863青年基金 ( 1998-187) ;中国科学院生命科学与生物技术青年科学家小组基金

摘　　要：对中国棉铃虫单粒包埋核多角体病毒 (HaSNPV)基因组中的HindⅢ Ⅰ片段的序列进行分析 ,该片段全长75 0 1nt,包括 10个开放阅读框 :AcMNPVORF111的同源基因 (Ac111) ,晚期表达因子 2 (lef 2 )基因 ,病毒核壳结构蛋白 p2 4基因、gp16 基因、多角体包膜蛋白 (polyhedronenvelopeprotein ,pep)基因、AcMNPVORF6 3的同源基因(Ac6 3) ,38 7kD基因 ,晚期表达因子 1(lef 1) 基因 ,及两个特有基因HaSNPV orf5 91和HaSNPV orf435。基因组的排列比较分析发现与AcMNPV有较大区别。In order to investigate the genomic organization of the single nucleocapsid nucleopolyhedrovirus(SNPV) of Helicoverpa armigera(HaSNPV),the HindⅢ Ⅰ fragment which is 7,501nt long and locates at map units(mu)12.3-17.9 of the viral genome was sequenced. The fragment contained ten open reading frames(ORFs)representing homologues of the AcMNPV orf 111 and orf 63,a lef 2 gene,a p24 gene,a gp16 gene,a pep gene,a 38. 7kD gene,a lef 1 gene and two unique genes named orf 591 and orf 435. Sequence alignment revealed that baculoviral P24,PEP and LEF 1 have some conserved motifs and gene structure as those observed in their homologues from other baculoviruses. The genomic arrangement of the ORFs in the HaSNPV HindⅢ Ⅰ fragment is very different from the arrangement of their homologues in the genome of Autographa californica multiple nucleocapsid(M)NPV.

关 键 词：中国棉铃虫单粒包埋核多角体病毒 S24基因 pep基因 lef-1基因 序列分析 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治] S476.13[农业科学—植物保护]