丝素膜上5-氟脲嘧啶渗透速度对pH值的应答  

Permeation of 5-Fluorouracil Thought Silk Fibroin Prbroin Protein Membrane Dependent on pH Value

作　　者：陈建勇[1] 刘冠峰[1] 沈之荃[2] 

机构地区：[1]浙江工程学院,纤维工程分院杭州310033 [2]浙江大学高分子科学与工程系

出　　处：《纺织学报》2001年第3期175-177,共3页Journal of Textile Research

基　　金：浙江省跨世纪学术和技术带头人培养资金资助项目

摘　　要：蚕丝丝素蛋白膜是一种具有弱碱性和弱酸性的两性荷电膜。在丝素膜－水溶液体系中，水溶液ｐＨ值变化会影响丝素膜荷电状况，进而影响到溶质的渗透速度和渗透量。５－氟脲嘧啶（５－ＦＵ）在外部溶液 ｐＨ＜７时透过丝素膜的速度基本上维持常量，当外部溶液ｐＨ＞８上下５－ＦＵ的渗透速度明显下降，表明在ｐＨ８上下５－ＦＵ的渗透状况呈开关状态。Permeation of 5-Fluorouracil through silk fibroin protein membrane was investigated. The membrane was prepared from Cocoon. Permeability coefficient of 5-FU was measure in the pH ranges from 3.0 to 9.0. The silk fibroin protein membrane was an amphoteric ion exchange membrane composed of both weak acid and weak base groups. The isoelectric point of this membrane was about pH 4.5. The measured results showed the permeability coefficient of 5-FU decreased about pH 8. It was due to 5-FU changed into anion 5-FU at alkaline solution. And the membrane was as cation exchanger membrane above pH 4.5. 5-FU anion was excluded by negatively charge group in the membrane. The permeation results of 5-FU were analyzed on the basis of TMS theory. According to theoretical analysis, the ion mobility of anion 5-FU in the membrane had distinctive changes at different pH, and apparent effective charge density in the membrane varied with pH changes. The permeate rate of 5-FU increased when pH change into 7, and decreased when pH return to 9, respectively.

关 键 词：蚕丝丝素膜 丝素蛋白 医学应用 5氟脲嘧啶 渗透速度 PH值 

分 类 号：R318.08[医药卫生—生物医学工程]