超广谱β-内酰胺酶检测的临床意义  

Clinical significance of measurement of extended spectrum β-lactamase

作　　者：徐雪松[1] 李秀[2] 孙景春[1] 金淑杰[1] 赵俊杰[1] 谢风[1] 迟迪[3] 

机构地区：[1]吉林大学中日联谊医院检验科,吉林长春130031 [2]吉林省人民医院检验科 [3]长春市中心血站

出　　处：《中国实验诊断学》2001年第3期112-113,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　研究近 3a临床分离的肺炎克雷伯菌和大肠埃希氏菌的耐药性及产超广谱 β 内酰胺酶 (ESBL)的比率。方法　应用双纸片协同试验筛选产超广谱 β 内酰胺酶的细菌 ,确正实验采用头孢他啶联合克拉维酸纸片扩散法。 结果　肺炎克雷伯菌、大肠埃希菌对亚胺培南的敏感率均为 10 0 % ,其次为头孢他啶分别为 71 7%和 91 1% ,产超广谱 β 内酰胺酶的比率分别为 2 8 3 %和 8 9%。结论　随着头孢三代抗生素等在临床应用的增多 ,临床分离的革兰阴性杆菌对头孢他啶等三代头孢的耐药性及产超广谱 β 内酰胺酶的比率呈上升趋势 ,准确的检测超广谱 βObjectives To study the prevalence of drug resistance and extended spectrum β lactamas in isolates of Klebsiella pneumoniae and Escherichia coli in recent 3 years. Methods\ Drug resistance was tested by NCCIS disk diffusion method The organisms were tested for ESBL production by double disk synergy and confirmed by combination with cefatazidime and clavulanic acid disk.Results The drug sensitivity of the isolates of Klebsiella pneumoniae and Escherichia coli to .Imipenem was 100%, to ceftazidime 71 7%,91 1% respectively.Conclusion The prevalence of drug resietance and extended spectrum β-lactamas in Gram Negative bacillus is rising.

关 键 词：肺炎克雷伯菌 大肠埃希氏菌 超广谱Β-内酰胺酶 耐药性 双纸片协同试验 

分 类 号：R446.5[医药卫生—诊断学]