5种药物对内毒素所致培养脐静脉内皮细胞能量代谢的影响  

THE CHANGE IN ENERGY CHARGE IN CULTURED HUVECS STIMULATED WITH LPS AFTER TREATMENT WITH FIVE CHINESE MEDICINES

作　　者：石富胜[1] 狄桂萍[1] 杨正国[1] 罗向东[2] 杨宗城[2] 吴旭[3] 

机构地区：[1]解放军第322医院,大同037006 [2]第三军医大学西南医院 [3]第三军医大学大坪医院

出　　处：《解放军医学杂志》2001年第6期429-431,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然基金重点课题! (编号 39830 40 0 )

摘　　要：为探讨山莨菪碱 (6 5 4 2 )、丹参、黄芪皂甙、β 七叶皂甙钠和蜕皮甾酮 5种药物干预后 ,对内毒素(LPS)所致培养脐静脉内皮细胞 (HUVECs)能量代谢的影响 ,建立LPS对体外培养的HUVECs致伤模型 ,测定培养的HUVECs能量代谢指标 (ATP ,ADP ,AMP ,EC)。结果显示 ,LPS与内皮细胞孵育后低浓度、早期内皮细胞被激活 ,随刺激时间的延长 ,培养HUVECs的EC降低 ,用上述 5种药物治疗后各时相点能量负荷明显增加 ,其中蜕皮甾酮、β 七叶皂甙钠、黄芪皂甙治疗效果优于 6 5 4 2、丹参。提示LPS对体外培养的HUVECs能量代谢有直接损伤作用 ,β 七叶皂甙钠、蜕皮甾酮、黄芪皂甙有一定的抗损伤作用 ,在体外培养的HUVECs能量代谢及能量储备方面优于 6 5 4 2。The protective function of five traditional herb medicines, namely anisodamine,escin,salvia miltiorrhizae radix,astragalus root and ecdysterone, in cultured HUVECs challenged by LPS. ATP,ADP,AMP and energy charge were measured in cultured HUVECs challenged by LPS after treatment with five herb medicines. The results showed that energy charge of HUVECs decreased after LPS challenge,and increased significiantly more afer the addition of escin,radix astragali and ecdysterone than other herbs.It suggested that escin, astragalus and ecdysterone can protect HUVECs from injury induced by LPS.

关 键 词：内毒素类 脐静脉 山莨菪碱 丹参 蜕皮甾酮 能量代谢 血管内皮细胞 烧伤 

分 类 号：R644[医药卫生—外科学]