类脂双层(BLM)概念及其实验进展——BLM的40年  

The Lipid Bilayer Concept and Its Experimental Realization

作　　者：毕只初[1] H.T.TIEN A.L.OTTOVA 

机构地区：[1]中国科学院化学研究所分子科学中心胶体和界面实验室,北京100080 [2]Membrane Biophysics Laboratory Department of Physiology

出　　处：《生物化学与生物物理进展》2001年第4期447-454,共8页Progress In Biochemistry and Biophysics

摘　　要：类脂双层研究的动力是来自生物世界 .虽然 196 1年首次报道生物体外的双层类脂膜 (BLMs)自组装 ,但从Hooke (16 72年 )起 ,实验科学家一直在处理有关BLM类型的界面吸附现象 .BLMs (或平面的类脂双层 )已应用在许多方面 ,即从基础的膜生物物理学到实际的爱滋病研究 ,从通过水光分解的太阳能转换 ,到应用支持双层类脂膜 (s BLMs)的生物传感器的发展 ,到包括细胞凋亡 (apoptosis)在内的光生物学 .综述了类脂双层概念的起源及其实验进展 ,以及BLMs用作某些生物膜模型的最新研究 .此外 ,简要描述近来有关通过sThe development of conventional BLMs (planar lipid bilayers) and later s BLMs and sb BLMs, have made it possible for the first time to study, directly, electrical properties and transport phenomena across a 5 nm ultrathin film separating two phases, in particular as models of biomembranes. As a result of extensive studies over the past 4 decades, biomembranes have now been recognized as the basic structure of Natures sensors and molecular devices. To impart relevant functions in BLMs, a variety of compounds such as ionophores, enzymes, receptors, pigments, tissues, etc. have been embedded. Some of these incorporated compounds cause the BLMs to exhibit non linear phenomena and photoelectric effects. The self assembled lipid bilayer, the most crucial component of all biomembranes, is in a liquid crystalline and dynamic state. Such a system, as we know intuitively, must act as some sort of a transducer capable of gathering information, processing it, and then delivering a response based on this information. Today, planar lipid bilayer research is a matured field of endeavor, as a result of applications of many disciplines and techniques including interfacial chemistry, electrochemistry, patch clamp techniques, spectroscopy, microelectronics, and others. In membrane reconstitution experiments, for example, the evidence is that intracellular signal transduction begins at membrane receptors. The research area covered in this paper is highly interdisciplinary. Emphasis has been placed on basic research. The past work has been benefited by a cross fertilization of ideas among various branches of sciences. The biomimetic approach to practical applications is unique and full of exciting possibilities. We can glean the design principles from Natures successful products and apply them to our research and development from which molecular medicine and advanced biosensors may ultimately depend.

关 键 词：双分子类脂膜 化学传感器 自组装 界面现象 重组 

分 类 号：Q73[生物学—分子生物学]