应用cDNA芯片分析79个新基因的人胚组织表达谱  

The 79 Novel Genes Expression Pattern in Human Fetal Tissues was Monitored by Using cDNA Microarray

作　　者：马淑华[1] 王敦成[2] 邹宗亮[1] 沈倍奋[2] 王升启[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《生物化学与生物物理进展》2001年第4期532-536,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金重点项目 (3 98890 0 1 ) .

摘　　要：大规模cDNA测序和生物信息学技术相结合 ,得到来自于商品化的人胚肾cDNA文库 79个代表新基因的表达序列标签 (EST) .随后 ,采用高速度机械手制备这些cDNA的基因芯片 ,用于鉴定 79个新基因的ESTs在2 0周、 2 6周两个胚胎时期 6种组织中的基因表达状况 ,以研究这些EST片段代表的新基因功能提供线索 .通过芯片杂交及结果分析 ,得到同一个组织两个不同时相 8个差异表达的基因 。79 EST fragments which represents corresponding novel genes were obtained by sequencing and bioinformatics analysis of human fetal kidney cDNA library. Microarray was prepared by using these novel EST fragments by automatic spotting. Expression patters of 79 ESTs of novel genes from human fetal kidney were analyzed in fetal brain and fetal heart tissues of 20-week-age and 26-week-age fetus by performing of cDNA chip hybridization. This provides clues for studying exact functions of the novel genes. 8 genes were obtained which were expressed differentially in the fetal brain and heart of 20-week-age and 26-week-age respectively. Then differentially expressed genes were identified by Northern analysis. The more exact function of the novel genes are under study.

关 键 词：CDNA芯片 新基因 表达序列标签 差异表达RNA分析 人胚组织表达谱 

分 类 号：Q754[生物学—分子生物学]