性类固醇激素及其受体在文昌鱼性腺和神经系统中的分布  

DISTRIBUTION OF SEX STEROID HORMONES AND THEIR RECEPTORS IN THE GONADS AND NERVOUS SYSTEM OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI)

作　　者：方永强[1] 翁幼竹[1] 胡晓霞[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《动物学报》2001年第4期398-403,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目 (No . 396 70 10 4)&&

摘　　要：首次用雌二醇、睾酮和孕酮受体的多克隆抗体在文昌鱼性腺、脑泡和神经管中进行免疫细胞化学定位研究。结果表明 ,不同发育时期的卵原细胞和卵母细胞中都存在雌激素、雄激素和孕激素受体。在小生长期 ,3种受体通常定位在卵原细胞和早期初级卵母细胞的胞质或核膜 ;在大生长期和成熟期 ,则定位在胞质和核质。在雄性 ,3种类固醇激素受体分布在不同发育时期的精原细胞、精母细胞和精子细胞 (雌激素受体例外 )中 ,受体阳性物定位在胞质和核。另外 ,用免疫细胞化学方法和性类固醇激素抗体对文昌鱼不同发育时期的性腺和神经系统进行研究 ,结果揭示 ,免疫阳性物存在于不同发育时期的卵巢和精巢中。在卵巢发育早期 ,卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应 ;在大生长期和成熟期 ,卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱 ,而对雌二醇则显著增强。在雄性 ,随着精巢发育成熟 ,精原细胞、精母细胞和精子细胞对睾酮抗体的免疫阳性反应逐渐增强。在大生长期和成熟期 ,精原细胞和精母细胞对孕酮抗体发生强的免疫阳性反应。在成熟期则对雌二醇抗体发生免疫阴性反应。精子始终为免疫阴性。本文研究还首次发现 ,文昌鱼脑泡和神经管中存在哺乳动物神经?Immunocytochemical localization were investigated for the first time in the gonads, brain vesicle and nerve tube of Amphioxus using polyclonal antibodies against 17β-estradiol, testosterone and progesterone receptors. The results indicated that the receptors of estrogen, androgen and progestogen existed in oogonia and oocytes of different developmental stages. At the small growth stage, three kinds of the sex steroid receptors usually were localized in the cytoplasm or nuclear membrane of oogonia and early primary oocyte, and localized in the cytoplasm and nucleoplasm of oocytes at the large growth stage and mature stage. In male, three kinds of sex steroid receptors were distributed in the spermatogonia, spermatocytes and spermatids (except estrogen receptor), and the positive reaction (substance) was localized in the cytoplasm and nucleus of these spermatogenic cells. In addition, the gonads at different stages and nervous system in amphioxus were also investigated by using immunocytochemical method and sex steroid hormones (17β-estradiol, testosterone and progesterone) antibodies. The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17β-estradiol, testosterone and progesterone antibodies. At the large growth stage and mature stage, immunopositive reaction to testosterone and progesterone antibodies in cytoplasm and nucleolar membrane of oocyte weakened obviously, but the immunopositive reaction to 17β-estradiol antibodies strenthened notably. In male, the immunopositive reaction in spermatogonia, spermatocyte and spermatid to testosterone antibodies strengthened gradually during the development and maturation of testis. At large growth stage and mature stage, the spermatogonia and spermatocyte showed strong immunopositive reaction to progesterone antibodies, but immunonegative r

关 键 词：文昌鱼 类固醇激素 受体 免疫细胞化学 性腺 神经系统 

分 类 号：Q571[生物学—生物化学]