水稻单株有效穗数和每穗粒数的QTL剖析  

QTL Dissection of Panicle Number Per Plant and Spikelet Number Per Panicle in Rice (Oryza sativa L.)

作　　者：徐建龙[1,2,4] 薛庆中[1] 罗利军[3] 黎志康[4] 

机构地区：[1]浙江大学农业与生物技术学院,杭州310029 [2]浙江省农业科学院原子能利用研究所,杭州310006 [3]中国水稻研究所 [4]国际水稻研究所植物育种,遗传与生化系

出　　处：《Acta Genetica Sinica》2001年第8期752-759,共8页

基　　金：国家973;863计划;国家自然科学基金(39893350);洛克菲洛基金资助项目&&

摘　　要：应用２９２个Ｌｅｍｏｎｔ／特青Ｆ１３重组自交系（ＲＩＬｓ）及其含２７２标记的遗传连锁图谱剖析单 株有效穗数（ＰＮ）和每穗粒数（ＳＮＰ）及其相关性状的遗传．所有性状呈现超亲分离，ＰＮ与ＳＮＰ 存在弱的负相关．检测到影响 ＰＮ和 ＳＮＰ及其相关性状的 ＱＴＬ ５１个和互作位点 ４５对，它们 可以解释 ６０％以上的性状总变异。 ＳＮＰ与其相关性状的 ＱＴＬ定位在一起，比较与 ＳＮＰ－ＱＴＬ 同一或相邻区域的 ＱＴＬ数，穗部枝梗数是长度性状的两倍，故前者对ＳＮＰ的作用更大。仅有 ２个ＰＮ－ＱＴＬ与ＳＮＰ相关性状的ＱＴＬ相邻，因此通过标记辅助选择有可能实现ＰＮ与ＳＮＰ的 有利重组。其中影响 ＰＮ的 ＱＰｎ４和影响穗枝梗数和长度的 ＱＰｂｎ３ａ，ＱＰｂｎ３和 ＱＰｂｌ４等主效 ＱＴＬ，在标记辅助选择中具有重要的应用价值。对通过标记辅助选择合理配置穗部性状 ＱＴＬ 产生新的高产穗型进行了讨论．The genetic mechanism underlying panicle number per plant (PN), spikelet number per panicle (SNP) and its related traits in rice was analysed using 292 F13 RILs from the cross of Lemont/ Teqing and a complete linkage map with 272 molecular markers. The RILs showed tremendous transgressive segregation for all traits studied. The weak negative correlation between PN and SNP was observed. Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified,collectively explaining over 60% of the total variation of individual traits. Almost all SNP-QTLs were attributable to one or more of its contributing components. Branching number traits had greater contributions to SNP than length traits, in which the first had twice as many QTLs mapped in the same or near regions with SNP as the latter.Only two PN-QTLs were mapped in the near regions with those of related traits of SNP, suggesting a reasonable recombination between PN and SNP would be available by marker-assisted selection (MAS). Some major QTLs including QPn4 for panicle number, QPbn3a, QPbn3b and QPbl4 for panicle branching and length would be of great value in MAS. It was discussed that a new high-yielding panicle type was resulted from reasonably deploying for QTLs of panicle traits by MAS.

关 键 词：单株有效穗数 每穗粒数 数量性状位点 上位性 水稻 标记辅助选择 QTL 

分 类 号：Q943[生物学—植物学]