Ralstonia eutropha CH34酯酶基因的克隆和序列分析  

CLONING AND SEQUENCING OF THE GENE ENCODING ESTERASE estA FROM RALSTONIA EUTROPHA CH34

作　　者：宋水山[1] Alexander Steinbchel 

机构地区：[1]河北省科学院生物研究所,石家庄050051 [2]Institut fr Mikrobiologie

出　　处：《微生物学报》2001年第4期402-407,共6页Acta Microbiologica Sinica

基　　金：德国教育科研部(BMBF)部长基金资助

摘　　要：采用含α 乙酸萘酯和固兰RR的表面琼脂法从RalstoniaeutrophaCH34的基因文库中筛选酯酶基因estA ,对含有estA的 1 7kbDNA片段的核甘酸序列分析表明 ,该基因全长82 5bp,编码由 2 75个氨基酸组成的EstA蛋白 ,分子量为 30 785D。经推导氨基酸序列的同源性分析 ,发现EstA与参与芳香化合物代谢中间位裂解途径的水解酶有很高的同源性。An esterase\|positive clone was isolated by screening a genomic library of Ralstonia eutropha CH34 constructed in Escherichia coli S17\|1 with top agar containing α\|naphtyl acetate and Fast Blue RR.A gne encoding esterase activity, estA was subcloned from this clone.Nucleotide sequencing of estA showed that it was a 825bp open reading frame,encoding an esterase EstA,composed of 275 amino acids with a predicted molecular mass of 30785 D.Homology analysis revealed that EstA exhibited significant amino acid similarity with the enzymes involved in the meta \|cleavage pathway in the metaboleism of aromatic acid compounds.

关 键 词：ReutrophaCH34 酯酶基因 DNA序列 大肠杆菌 克隆 表达 

分 类 号：Q785[生物学—分子生物学]