根瘤菌吸氢酶基因转移的研究  

STUDIES ON TRANSFERENCE OF HYDROGENASE GENES OF RHIZOBIUM ARACHIS

作　　者：王振宇[1] 龙敏南[1] 刘月英[1] 张风章[1] 许良树[1] 朱天赐 蒋水柳 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005 [2]厦门市同安汀溪灌溉试验站,厦门361100

出　　处：《微生物学报》2001年第4期421-426,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金! (3 9770 0 70 );福建省自然科学基金! (B 991 0 0 0 1 )&&

摘　　要：通过三亲本杂交将含有花生根瘤菌吸氢基因的质粒pZ 55(Tcr)转入不吸氢的花生根瘤菌Ra 34等菌株 (Hup- ,Nif+,Apr)中 ,筛选到既具有吸氢又具有固氮能力的花生根瘤菌结合株Rz34 2。在自生和共生条件下 ,结合株均可表达高吸氢和高固氮活性。以结合株Rz34 2接种的花生植株叶片的干重比不接种的、接种受体株Ra34(Hup- )和接种对照菌株L8 3(Hup+,Nif+)的分别高 6 2 %、7 6%和 6 3% ;种子的含氮量分别高 8 9%、1 0 0 %和 6 0 % ;产量分别高 1 8 8%、1 0 5%和 1 0 7%。研究结果表明 ,以含吸氢基因的结合株接种花生能提高根瘤菌与花生的共生固氮效率 ,增加作物的产量。The hydrogen\|uptake genes were transferred into wild Rhizobium arachis Ra strains (Hup -,Nif +,Ap r)by triparental mating using pRK2013 as help plasmid.A transconjugant R.arachis Rz34\|2(Hup +,Nif +,Ap r,Tc r)which expressed high activities of hydrogenase and nitrogenase under free\|living and symbiotic state was screened.Peanut inoculation test with recipient R.arachis Ra34,transcojugant Rz34\|2 and control strain R.arachis L8\|3 (Hup +,Nif +)was carried out respectively.The results showed that,compare to treatment without inoculation\,inoculation with R.arachis Ra34 and R.arachis L8\|3,the dry weight of leaf inoculated with transconjuant Rz34\|2 increased 6.2%\,7.6% and 6.3% respectively;the N\|content of seed increased 8.8%\,10.0% and 6.0%;the output increased 18.8%\,10.5% and 10.7%.This suggested that legume plants inoculated with Rhizobium strains (Hup +)were more efficient to accumulate N and to increase its output.

关 键 词：花生根瘤菌 转吸氢基因 大田实验 吸氢酶 固氮酶 基因转移 

分 类 号：Q933[生物学—微生物学]