费氏中华根瘤菌内源质粒的不相容性及其在质粒消除中的应用  

INCOMPATIBILITY AMONG INDIGENOUS PLASMIDS OF SINORHIZOBIUM FREDII STRAINS AND ITS APPLICATION FOR PLASMID CURING

作　　者：缪礼鸿[1] 周俊初[1] 

机构地区：[1]农业部农业微生物重点实验室,华中农业大学武汉430070

出　　处：《微生物学报》2001年第4期432-439,共8页Acta Microbiologica Sinica

基　　金：欧盟INCO-DC合作研究计划ERBIC18CT970191课题经费资助

摘　　要：用Tn5 Mob sacB转座子标记费氏中华根瘤菌 (Sinorhizobiumfredii)HN0 1和WWG1 8的内源质粒 ;在含有 1 0 %蔗糖的TY培养基上进行了质粒消除试验以鉴定其功能。将HN0 1的标记共生质粒pSymHN0 1b转入费氏中华根瘤菌WWG1 8SR和C361SR中 ,质粒快速检测、质粒消除和植物盆栽结瘤试验结果证明 :HN0 1的共生质粒与WWG1 8SR的共生质粒具有不相容性 ,但能与其非共生质粒相容。相反 ,HN0 1的共生质粒可与C361SR的共生质粒相容 ,但与其中一个非共生质粒具有不相容性。利用费氏中华根瘤菌不同菌株质粒间的不相容性 ,本研究成功地消除了WWG1 8SR的共生质粒和C361SR的一个非共生质粒。The indigenous plasmids of S.frediii HN01 and WWG18 were labeled with Tn5\|Mob\| sacB .Those plasmids were cured on TY medium containing 10% sucrose to identify its function.The pSymHN01b of HN01 was transferred into WWG18SR and C361SR.The results of plasmid detection,curing and pot plant experiment revealed that the incompatibility was existed between pSymHN01b and symbiotic plasmid of WWG18SR or the third plasmid of C361SR.But it was compatible with the nonsymbiotic plasmid of WWG18SR and symbiotic plasmid of C361SR.Using plasmid incompatibility,The symbiotic plasmid of WWG18SR and one nonsymbiotic plasmid of C361SR were eliminated successfully.

关 键 词：费氏中华根瘤菌 质粒 不相容性 

分 类 号：Q939.11[生物学—微生物学]