费氏中华根瘤菌(Sinorhizobium fredii)的多样性研究  

Diversity of Sinorhizobium fredii strains.

作　　者：陈明周 谢福莉[1] 周俊初[1] 

机构地区：[1]华中农业大学农业部农业微生物重点实验室,武汉430070

出　　处：《应用生态学报》2001年第4期597-600,共4页Chinese Journal of Applied Ecology

基　　金：欧盟INCO DC资助项目 (ERBIC970 1 91 )

摘　　要：用大豆品种Willimas和黑龙 33从多年种植大豆未接种根瘤菌的土壤中捕集大豆根瘤菌 ,从分离株中选出 5 0株费氏中华根瘤菌 ,对供试菌株的培养特性、生长速度、耐酸、耐碱性、生长最终pH值、天然抗药性、CN源利用、刚果红吸收强度、产黑色素能力和质粒图谱类型进行了系统的比较研究 ,并通过聚类分析得到树状图谱 ,证实了不同土壤中费氏中华根瘤菌的多样性 .Rhizobial strains were isolated from soils growing with soybean cultivar Heilong 33 and Willimas.Fifty Sinorhizobium fredii strains were chosen,and their biological characteristics including growth velocity,acid-alkali endurance,resistance of intrinsic antibiotics,utilization of carbon and nitrogen sources,absorption of congo red,ability of melanin production,and plasmid profiles were comparatively researched.The dendrogram was described using the method of cluster analysis,and the biodiversity of Sinorhizobium fredii from different soils was proved.

关 键 词：费氏中华根瘤菌 多样性 聚类分析 

分 类 号：Q945[生物学—植物学]