当归对高脂血清所致ECV_(304)细胞损伤的保护作用  

Protective effect of Angelica on ECV_(304) from injury induced by hyperlipidemic serum in vitro

作　　者：王保华[1] 欧阳静萍[1] 刘永明[1] 魏蕾 杨静薇[1] 

机构地区：[1]湖北医科大学病理生理教研室,武汉430071

出　　处：《生理学报》2001年第3期240-243,共4页Acta Physiologica Sinica

摘　　要：实验观察了高脂血清对培养的人脐静脉内皮细胞（ＥＣＶ３０４）的损伤及传统中药当归的保护作用，以探讨当归的抗动脉粥样硬化作用及其可能机制。培养人脐静脉内皮细胞，以高脂血清作损伤因子，用扫描电镜观察细胞的超微结构，分光光度法检测细胞培养液中一氧化氮（ＮＯ）的含量，免疫细胞化学方法检测细胞表面细胞间粘附分子－１（ＩＣＡＭ－１）、碱性成纤维细胞生长因子（ｂＦＧＦ）及转化生长因子β１（ＴＧＦβ１）的表达。与高脂血清孵育２４ｈ后，内皮细胞的超微结构明显收损，且细胞表面ＩＣＡＭ－１、ｂＦＧＦ的表达明显增加，而细胞培养液中ＮＯ的量及细胞表达ＴＧＦβ１明显减少。加入当归后，高脂血清对内皮细胞的这些作用均可被逆转。当归对内皮细胞中ＩＣＡＭ－１、ｂＦＧＦ、ＴＧＦβ１及ＮＯ表达改变的影响可能与其抗动脉粥样硬化的作用有关。The aim of this article was to examine the protective effect of Chinese traditional medicine An-gelica on human umbilical vein endothelial cells (HIUVECs, ECV304) from injury induced by hyperlipidemic serum (HLS) and to study the underlying mechanisms. Microstructures of HUVECs were observed by a scan-ning electron microscope. Spectrophotometer and immunocytochemical methods were used to detect the content of NO in the suspension and expression of ICAM-1, TGFβ1, bFGF on the cell surface, respectively. After be-ing incubated with HLS for 24 hours, HTJVECs exhibited pronounced morphological changes, such as disap-pearance of microvilli on the endotheial cell (EC) surface, rupture of cell membranes, etc. Expression of Ⅰ-CAM-1 and bFGF in ECs was significantly increased, while expression of TGFβ1 and the release of NO from ECs were significantly decreased. All these effects of HLS on ECs can be reversed by Angelica significantly. The above effects of Angelica may be related to its anti-atherosclerotic action.

关 键 词：当归 血管内皮细胞损伤 超微结构 ICAM-1 NO BFGF 动脉粥样硬化 高脂血 

分 类 号：R285.5[医药卫生—中药学]