微生物絮凝剂的研制——菌种选育、絮凝效果及提取工艺  

SCREENING FOR AND CHARACTERISTICS OF MICROBIAL FLOCCULANT

作　　者：叶晶菁[1] 谭天伟[2] 

机构地区：[1]北京化工研究院,北京100013 [2]北京化工大学,北京100029

出　　处：《微生物学通报》2001年第4期31-35,共5页Microbiology China

基　　金：中国石油化工股份有限公司科学技术研究开发资金资助项目 (No . 990 0 1 8)

摘　　要：从土壤、河泥、活性污泥中分离出 75 2株细菌 ,以发酵液对高岭土悬浮液絮凝效果　　 　中国石油化工股份有限公司科学技术研究开发资金资助项目 (No . 990 0 1 8)收稿日期 :2 0 0 0 0 4 0 3 ,修改日期 :2 0 0 0 1 2 3 0为指标 ,筛选出 1株絮凝剂产生高效菌。该菌在实验室培养条件下 ,以 0 2mL/3 0mL接种量 ,5h种龄的种子液接种 ,2 5℃、pH7摇床培养 3d可达最高絮凝活性。最佳培养基配方为 :葡萄糖 2 0g ,尿素 0 3g ,酵母膏 0 6g ,NaCl0 6g ,K2 HPO4·3H2 O6g ,KH2 PO43g,pH7;微生物絮凝剂的絮凝效果受加样量、pH、金属离子、温度、搅拌速度、水质等多种因素的影响。实际废水试验显示 ,该菌对去除水中悬浮物能力较强 ,且对高浓度染料废水的脱色能力较好 ;采用水抽提 丙酮沉淀 真空干燥的工艺路线 ,最终从 1 0 0mL发酵液中得到0 1 985g干品。strain 49 #(BD-4),which had the highest flocculating activity among 752 strains,was picked out.The fermentation condi tions and the flocculating influence factors were studied.By testing,this cult ure broth had a much more ability to get rid of suspended materials in water and to decolourize the high-density dyestuff wastewater. In laboratory scale,bioflocculant was roughly extracted from culture broth by t he technological process,water extraction-organic solvent sediment-vacuum dry .The suitable work conditions of each step were set up through the tests.0 198 5g dry product was obtained from 100mL culture broth.

关 键 词：微生物絮凝剂 菌种选育 废水处理 提取 

分 类 号：Q93-3[生物学—微生物学]