电子供体及受体对棕色固氮菌抗氨阻遏能力的影响  

EFFECT OF ELECTRON DONOR AND ACCEPTOR ON CAPACITIES OF AMMONIA-RESITANT FROM AZOTBACTER VINELANDII

作　　者：黄河清[1] 孙耀东[1] 林庆梅[2] 唐华 翟文景 

机构地区：[1]厦门大学生物学系 [2]厦门大学环境科学研究中心,厦门361005

出　　处：《微生物学通报》2001年第4期55-60,共6页Microbiology China

基　　金：福建省自然科学基金资助项目 (No . 960 0 6)

摘　　要：电子供体连二亚硫酸钠、甲基紫精及电子受体亚甲蓝均能强烈抑制棕色固氮菌表达固氮活性 ,并引起该菌的抗氨阻遏能力减弱。适当提高氧压 ,能提高菌体的固氮活性近1 5 % ,但过高的氧分压反而抑制菌体的固氮活性。此外 ,提高氢分压能降低棕色固氮菌菌体内的还原电位 ,从而达到提高菌体抗氨阻遏能力的效果。Electronic donors dithionite as well as m et hyl viologen and it receptor methylene blue strongly inhibited activity of nitro gen fixation that Azotobacter vinelandii expressed,which ca used capacity of ammonia-resistant weaken greatly.About 15% of total activity f rom the whole cell were risen by increasing suitable oxygen atmosphere.Opposite, t he activity was inhibited by increasing over concentration of O 2 further.The r eduction potential in the inside environment from Azotobacter vinelan dii whole cell shifted to negative by increasing hydrogen pressure,whi ch resulted in its capacity of ammonia-resistant rose.

关 键 词：棕色固氮菌 抗氨阻遏 内环境电位 固氮活性 电子供体 电子受体 

分 类 号：Q814[生物学—生物工程]