Nd:YAG激光辐照花生种子的生物学效应  

Biological Effects of Laser Irradiation on Peanut Seeds

作　　者：欧琳[1] 陈荣[1] 林彤[1] 曾雅娟[2] 肖华山[2] 庄伟建[3] 蔡来龙[3] 

机构地区：[1]福建师范大学物理系,福州350007 [2]福建师范大学生物系,福州350007 [3]福建农业大学作物科学院,福州350006

出　　处：《光电子．激光》2001年第7期751-753,共3页Journal of Optoelectronics·Laser

基　　金：福建省自然科学基金资助项目 (F9810 0 11)

摘　　要：本文研究了用 Nd:YAG(10 60 nm)激光辐照花生种子后其 F1 代的细胞生物学效应和农艺性状变异。研究结果表明 ,在功率密度为 35 .84W/ cm2情况下 ,辐照时间由 3s增至 8s均能诱发根尖细胞的染色体畸变 ,畸变率相应由 0 .16%增至 0 .5 3% ,染色体畸变类型也随之增加 ;8s处理后的染色体畸变类型中 ,中期提早分离、数目变异、粘连、不均等分裂、多极分裂及核向细胞质突起等在 3s处理中均无出现。根据种子萌发和生长情况 ,35 .84W/ cm2 × 8s处理已达到致死剂量。3s处理组的 F1 植株的主茎高、单株结果数。After Nd:YAG laser irradiating on dry seeds of peanut at 1 060 nm,cytological effects and agro character in the F 1 generation have been studied in the present paper.The results showed there was chromosomal aberration of peanut tip cell induced with laser 35.84 W/cm 2 power density.With irradiating time added from 3 s to 8 s the rate of aberration increased from 0.16% up to 0.53%,and the types of chromosomal aberration also increased.Many aberration types,such as earlier separated mitosis metaphase,variability of number,sticky,non equal division,polypolarity division,nucleus protrudes to cytoplasm and so on were found in 8 s treatment but not in 3 s treatment.However,the dose of 35.84 W/cm 2×8 s leads to lethal dose according to the seeds sprouting and growing.On the seeds with 35.84 W/cm 2×3 s treatment,mine stem height,total pods per plant,mature pods per plant and pods weight per plant were more than the seeds unirradiated.

关 键 词：激光辐照 花生种子 生物学效应 ND:YAG激光 

分 类 号：R318.51[医药卫生—生物医学工程]