地塞米松对家兔失血性休克-再灌注损伤的防治作用  

Protective effects of dexamethasone on reperfusion injury after hemorrhagic shock in rabbits

作　　者：林丽娜[1] 王万铁[2] 徐正衸[2] 李东[3] 

机构地区：[1]温州医学院附属第一医院麻醉科,浙江温州325003 [2]温州医学院基础学院病生教研室 [3]温州医学院检验系分析中心

出　　处：《中国危重病急救医学》2001年第6期362-364,共3页Chinese Critical Care Medicine

基　　金：浙江省教育厅科研基金;温州市"5 5 1人才工程"培养基金!联合资助项目 (No.980 18)

摘　　要：目的 :探讨地塞米松对失血性休克再灌注损伤的防治作用。方法 :制备家兔失血性休克模型 ,随机分为地塞米松保护组 ( 组 )和未用地塞米松对照组 ( 组 ) ,检测血浆和组织一氧化氮代谢产物 (NOP)、丙二醛(MDA)含量及平均动脉压 (MAP)。结果 :休克前 2组动物 NOP、MDA及 MAP间均无统计学差异。休克 90分钟时 2组动物 NOP、MDA均明显升高 ,MAP均显著下降。再灌注后 , 组 NOP及 MDA均逐渐下降 ,再灌注 3小时后接近休克前水平 ,但明显低于休克 90分钟和 组同时间点水平 ; 组 MAP逐渐上升 ,再灌注 3小时后接近休克前水平 ,但明显高于休克 90分钟和 组同时间点水平。此外 , 组心、肺、肝、肾、肠道组织 NOP及MDA含量均明显低于 组。结论 :地塞米松可降低一氧化氮及氧自由基水平 ,减轻脂质过氧化反应 。Objective:To investigate the protective effect of dexamethasone (DXM) on reperfusion injury induced by hemorrhagic shock in rabbits.Methods:U sing a hemorrhagic shock model,2 0 rabbits were random ly divided into non treatm ent group (group ) and treatm ent group (group ) ,10 rabbits in each group. Rabbits in group DXM(2 0 mg/ kg) was intravenously administrated imm ediately after reperfusion,while rabbits of the group received the sam e volum e of normal saline.Plasm a and tissue nitric oxide products(NO, NO- 2 / NO- 3) ,malonyldialdehyde(MDA) and m ean arterial pressure(MAP) were m easured before shock,90 minutes of shock and 6 0 ,12 0 ,180 m inutes after reperfusion,respectively.Results:Baseline measurements of NO,MDA and MAP were similar in two groups.90 minutes after hem orrhage,plasma NO and MDA contents significantly increased and MAP values remarkably decreased in both groups as compared to baseline values (all P<0 .0 1vs.baseline) .After treatm ent with DXM,NO and MDA levels gradually decreased and had a tendency approaching baseline values at3hours after reperfusion,which were significantly lower than that of 90 m inutes hem orrhage and group .In group ,MAP increased after reperfusion,at 3hours,and it was markedly higher than that of 90 m inutes hemorrhage and group .In addition,NO and MDA levels in the heart,lung,liver,kidney and intestine in group were lower than those in group .Conclusions:DXM could effectively protect vital organs from reperfusion injury following hemorrhagic shock by reducing levels of NO and oxygen free radicals,attenuating lipid peroxidation.

关 键 词：失血性休克 缺血-再灌注损伤 一氧化氮 氧自由基 地塞米松 

分 类 号：R605.971[医药卫生—急诊医学] R364.1[医药卫生—外科学]