我国分离的两株病毒为重组甲病毒  

Two virus strains of isolated in China are recombinant alphaviruses

作　　者：何海怀[1] 吕新军[2] 杨益良[1] 付士红[1] 周国林[1] 张桂筠[2] 陈向伟[2] 梁国栋[1] 金奇[1] 侯云德[1] 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室,北京100052 [2]山西医科大学

出　　处：《中华实验和临床病毒学杂志》2001年第2期120-124,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金优秀国家重点实验室项目资助(3982 30 0 2 )

摘　　要：目的　明确我国分离的XJ 90 2 6 0和XJ 910 0 6病毒的分类地位、种系发生和遗传型。方法　特异引物逆转录 聚合酶链反应 (RT PCR)扩增两株病毒的NSP4、E1基因区和 3′端非编码区 ,测序 ,进行核苷酸序列同源性比较和 3′端非编码区核苷酸序列分析 ,并结合同属其他病毒这些基因区的核苷酸序列进行种系进化分析。结果　两株病毒的核苷酸序列同源性为 10 0 % ,与西方马脑炎病毒的核种酸序列同源性最高 ,具有西方马脑炎病毒 3′端非编码区结构特征 ,其NSP4基因区与东方马脑炎病毒同源 ,E1基因区与辛德毕斯病毒同源 ,两株病毒均位于西方马脑炎病毒B组 ,与西方马脑炎病毒俄罗斯分离株进化关系最近。结论　我国分离的XJ 90 2 6 0和 910 0 6病毒属于西方马脑炎病毒同一遗传型 ,均为重组甲病毒。Objective To dentermine the classification,phylogenetic and genetic type of XJ\|90260 and XJ\|91006 viruses isolated in China.Methods Two pairs of specific primers were designed to amplify the NSP4 and E1\|3′ UTR genes of XJ\|90260 and XJ\|91006 viruses and the nucleotide sequenses between them and with that of other Alphavirus were compared.Results The nucleotide sequenses identity between XJ\|90260 and XJ\|91006 viruses was 100% and has showed the highest with WEE,comparing with the other 26 Alphaviruses in 3′ UTR.NSP4 gene of XJ\|90260 and XJ\|91006 viruses high homologous with EEE virus and E\|1 gene high homologous wih SIN virus.XJ\|90260 and XJ\|91006 viruses belong to the B group of WEE and are closest to the Russian strain(Y62\|33) by phylogenetic analysis.Conclusion XJ\|90260 and XJ\|91006 viruses isolated in China are recombinant Alphaviruses,they belong to the same genetic type as WEE does.

关 键 词：披膜病毒科 甲病毒属 核苷酸类/分析 种系发生/分析 西方马脑炎病毒 

分 类 号：R373[医药卫生—病原生物学]