HCV准种多样性及其与临床关系的研究  

Clinical implications of HCV quasispecies heterogeneity in patients with hepatitis C

作　　者：唐小平[1] 钱可平[2] Johnson YNLAU 袁小珍[1] 

机构地区：[1]广州市第八人民医院传染病研究所,广州510060 [2]美国佛罗里达大学医学院肝病中心

出　　处：《中华实验和临床病毒学杂志》2001年第2期173-175,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解丙型肝炎病人丙型肝炎病毒 (HCV)准种感染情况及其与临床的关系。方法　采用针对HCVE2高变区 1(HVR1)的单链构象多肽性分析法 (SSCP) ,对 76例丙型肝炎患者血清HCV准种进行检测。结果　 72例患者 (94 7% )HVR1PCR阳性 ,SSCP条带数为 2～ 11条 ,平均 5 8条。准种数目急性肝炎 (3 1± 1 2 ) <慢性肝炎 (6 0± 2 3) <肝硬化和 或原发性肝癌 (8 4± 4 3) (P <0 0 1)。经输血及静脉吸毒感染的丙型肝炎患者HCV准种数量显著多于经其他途径 (散发 )感染的丙型肝炎患者 (P <0 0 5 )。HCV1a、1b基因型感染者准种数量显著多于HCV2、3型感染者 (P <0 0 5 )。准种数目与HCV病毒血症水平显著相关 (P <0 0 1)。结论　SSCP是一种简便、快速、可靠的HCV准种分析方法 ;HCV准种多样性与丙型肝炎的慢性化及疾病进展有关 ;感染时间长、基因 1型感染及高水平的血清HCVRNA水平预示体内存在较多的HCV准种。Objective To determine the heterogeneity of viral quasispecies and its clinical significance in patients with hepatitis C. Methods Quasispecies in the sera from 76 patients infected with hepatitis C virus were detected using single stranded conformational polymorphism (SSCP) analysis of the HCV E2 hypervariable region 1(HVR1).Results HVR1 was amplified in 72 (94.7%) of the 76 patients. The average number of SSCP bands was 5.8, with a range from 2 to 11. The numbers of quasispecies in acute hepatitis, chronic hepatitis and liver cirrhosis and/or primary heptocellular carcinoma were 3.1±1.2,6.0±2.3 and 8.4±4.1,respectively. There was a statistically significant difference among them ( P <0.01). Patients with infection acquired by blood transfusion and I.V. drug use had greater number of quasispecies than those acquired by other transmission pathway (sporadic) ( P <0.05). Patients with genotype 1 a and 1 b infection had increased quasispecies compared with those infected with HCV type 2 and 3( P <0.05). Increased quasispecies heterogeneity was significantly correlated with serum HCV RNA levels ( P <0.01). Conclusions SSCP is a simple, rapid and reliable method for the analysis of HCV viral quasispecies heterogeneity. Quasispecies heterogeneity plays an important role in HCV persistent infection and in the progress of hepatitis C. The duration of HCV infection, HCV genotype and HCV viremia are important determinants for the evolution of HCV quasispecies heterogeneity.

关 键 词：丙型肝炎病毒 准种 单链构象多肽性分析 病毒血症 

分 类 号：R373[医药卫生—病原生物学]