豚鼠发育过程中前庭上皮IGF-1及IGF-1R的表达变化  

Expression and Function of IGF-1 and IGF-1R in Vestibular Epithelium During the Development of Guinea Pig

作　　者：胡金玮[1] 王锦玲[1] 黄维国[1] 姜鸿彦[1] 邱建华[1] 乔莉[1] 

机构地区：[1]第四军医大学西京医院耳鼻咽喉科,西安710032

出　　处：《听力学及言语疾病杂志》2001年第3期129-132,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目(项目编号 39870788)

摘　　要：目的检测胰岛素样生长因子－ｌ（ｉｎｓｕｌｉｎ－ｌｉｋｅ　ｇｒｏｗｔｈ ｆａｃｔｏｒ－１，ＩＧＦ－ｌ）及其受体（ｉｎｓｕｌｉｎ－ｌｉｋｅ ｇｒｏｗｔｈ　ｆａｃｔｏｒ－１ ｒｅｃｅｐｔｏｒ，ＩＣＦ－１Ｒ）在豚鼠发育过程中前庭上皮的表达和分布特点，并探讨其生物学特性及意义。方法　利用免疫组织化学的方法，以ＩＧＦ－１和ＩＣＦ－１Ｒ抗体为标记物，检测发育期豚鼠前庭器中的表达情况及分布特点，以增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｏｎ ｃｅｌｌ ｎｕｃｌｅａｒ ａｎｔｉｇｅｎ，ＰＣＮＡ）检测细胞增殖变化。结果ＩＧＦ－１及ＩＣＦ－１Ｒ可在豚鼠前庭上皮细胞中表达，随年龄增加而逐渐减少；ＰＣＮＡ表达于支持细胞，随年龄增加，阳性细胞数逐渐减少。结论　豚鼠正常发育过程中前庭上皮中有ＩＣＦ－ｌ及ＩＧＦ－１Ｒ的表达，为内源性的ＩＣＦ－ｌ通过旁分泌和自分泌方式在局部发挥其功能，壶腹嵴感觉上皮ＩＧＦ－１及ＩＧＦ－１Ｒ表达与细胞增殖相同步，ＩＧＦ－１在发育早期是强烈的有丝分裂原，促进细胞增殖、分化。Objective The expression of IGF-1 and IGF-1R of vestibular epithelium in normal developing guinea pig was investigated to elucidate the function and biological character of IGF-1. Methods Guinea pigs of different developing ages were studied. The expression of IGF-1 and its receptor was detected by anti-IGF-1 and anti-IGF-1R, while eell proliferation was detect- ed by proliferation cell nuclear antigen(PCNA) immunocytochemistry. Results Immuno - reactivity to IGF-1 and its receptor was detected in the vestibular sensory epithelium of guinea pigs, and the quantity of staining decreased with aging. PCNA staining de- tected in supporting cells, and the positive cells decreased with aging. Conclusion IGF-1 and its receptor were expressed in vestibular sensory epithelium, and IGF-1, in concordance with cell proliferation, might be the endogenous insulin - like activity as a paracrine or autocrine manner during the normal ear development of guinea pig. IGF-1 played multiple roles during ear develop- ment, including regulation of proliferation and differentiation in early stage.

关 键 词：前庭感觉上皮 胰岛素样生长因子-1 胰岛素样生长因子-1受体 细胞增殖 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]