应用HRP逆行示踪技术评价兔颞骨内面神经缺损的修复  

Application of HRP Retrograde Tracing for the Assessment of Intratemporal Facial Nerve Regeneration with Chitin Chamber in Rabbit

作　　者：蔡萍[1] 刘公汉[1] 华清泉[1] 吴展元[1] 肖伯奎[1] 

机构地区：[1]武汉大学人民医院耳鼻咽喉-头颈外科,武汉430060

出　　处：《听力学及言语疾病杂志》2001年第3期140-141,共2页Journal of Audiology and Speech Pathology

基　　金：湖北省教育委员会青年基金资助课题

摘　　要：目的　探讨几丁质室修复颞骨内面神经缺损神经纤维的再通以及与神经元胞体的连续性。方法用几丁质室修复兔左侧颞骨内面神经缺损，术后１、３和５个月切断颞外段面神经主干，将辣根过氧化物酶（ｈｏｒｓｅｒａｄｉｓｈ ｐｅｒｏｘｉｄａｓｅ，ＨＲＰ）涂于其近端，同法标记正常神经对照组，观察脑桥下段的神经元细胞。结果术后３个月在同侧腹外侧面神经运动核区出现数量不等的标记细胞，术后５个月数量增多，与正常神经的神经元胞体来源相同。结论　ＨＲＰ可沿再生神经纤维逆行运输，表明神经纤维再通，周围支到中枢的解剖通路重建。Objective To investigate the regeneration of intratemporal facial nerve gap and its continuity with facial nerve nucleus slier chitin chamber bridge. Methods Rabbit intratemporal facial nerve gap was repaired with chitin chamber. The facial nerve below the stylomastoid foramen was amputated. The proximal end was traced retrogradely with horseradish peroxidase (HRP). Normal facial nerve was traced with the same method. The histological examination of inferior pen was performed. The results were observed at 1, 3 and 5 months after operation. Results The tracing showed that the labeled cells were located in ip- silateral ventrolateral part of pon, which was the site of normal facial nerve nucleus. There were more labeled cells at 5 months af- ter operation than that at 3 months. Conclusion Horseradish peroxidase could be transported along the regenerated nerve fibers, which suggested that not only the proximal transected neural stump connected with the distal transected neural stump within chitin chamber, but also the peripheral part of facial nerve connected with the central part.

关 键 词：HRP逆行示踪 颞骨内面神经 神经再生 几丁质 神经元 

分 类 号：R764.4[医药卫生—耳鼻咽喉科]