内淋巴囊和肾小管上皮细胞透明质酸合成酶mRNA的表达  

The Expression of Hyaluronan Synthase mRNA in the Endolymphatic Sac Cells and Renal Tubules

作　　者：牟忠林[1] 刘兆华[2] 陈剑[2] 

机构地区：[1]重庆医科大学第二附属医院耳鼻咽喉科,重庆400010 [2]第三军医大学大坪医院耳鼻咽喉-头颈外科

出　　处：《听力学及言语疾病杂志》2001年第3期156-157,共2页Journal of Audiology and Speech Pathology

基　　金：重庆市应用基础研究项目(渝科委计1999年33号)资助

摘　　要：目的　用寡聚核苷酸探针检测内淋巴囊和肾小管上皮细胞透明质酸合成酶ｍＲＮＡ表达的异同。方法　通过查阅核酸序列数据库，自行设计并合成了透明质酸合成酶寡聚核苷酸探针，应用原位杂交技术检测透明质酸合成酶ｍＲＮＡ在豚鼠内淋巴囊和肾小管上皮细胞的表达。结果　在内淋巴囊近侧端、中间部和肾小管部分上皮细胞胞浆显示透明质酸合成酶ｍＲＮＡ的阳性表达，但近曲小管的阳性细胞百分数稍多。结论　不但扩大了核酸杂交的应用范围，还为内淋巴囊和肾小管上皮细胞对水电解质平衡可能都具有透明质酸／透明质酸酶的双重调节作用提供了理论依据。Objective To study the difference between the mRNA expressions of hyaluronan synthase in the epithelial cells of endolymphatic sac and renal tubules with the oligonucleotide probe. Methods The oligonucleotide probe of hyaluronan synthase was designed and synthesized, the mRNA expressions of hyaluronan synthase in the epithelial cells of endolymphatic sac and renal tubules were detected with hybridization in situ. Results mRNA of hyaluronan synthases were strongly expressed in some epithelial cells of endolymphatic sac and renal tubules. Conclusion It is confirmed that a dual regulatory system for hyaluronan /hyaluronidase exists in the epithelial cells of endolymphatic sac and renal tubules at the level of molecular biology.

关 键 词：内淋巴囊 肾小管 透明质酸合成酶 原位杂交 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]