氯沙坦对血管平滑肌细胞内单核细胞趋化因子-1表达的影响  

Effects of Angiotensin Ⅱ on Protein and mRNA Expression of Monocyte Chemoattractant Protein-1 in Cultured Rabbit Aortic Smooth Muscle Cells

作　　者：李爱民[1] 何作云[1] 周小波[1] 覃军[1] 耿建萌[1] 

机构地区：[1]第三军医大学新桥医院心内科,重庆市400037

出　　处：《中国动脉硬化杂志》2001年第2期108-111,共4页Chinese Journal of Arteriosclerosis

摘　　要：为了探讨血管紧张素Ⅱ受体拮抗剂氯沙坦对血管平滑肌细胞内单核细胞趋化蛋白 1表达的影响 ,以培养幼兔主动脉平滑肌细胞为研究对象 ,分别给予不同浓度血管紧张素Ⅱ和 或血管紧张素Ⅱ受体拮抗剂氯沙坦 ,采用免疫组织化学、原位杂交与酶联免疫吸附技术检测不同处理组平滑肌细胞内单核细胞趋化蛋白 1蛋白及其mR NA表达和平滑肌细胞培养介质中单核细胞趋化蛋白 1含量的变化。结果发现 ,10 - 6 ～ 10 - 1 0 mol L血管紧张素Ⅱ呈剂量依赖性地增加平滑肌细胞内单核细胞趋化蛋白 1蛋白及其mRNA表达水平 ,增高培养介质中单核细胞趋化蛋白 1蛋白含量 (P均 <0 .0 0 1)。 10 - 5 ～ 10 - 7mol L氯沙坦预处理使血管紧张素Ⅱ刺激的平滑肌细胞内单核细胞趋化蛋白 1蛋白及其mRNA表达水平及培养介质中单核细胞趋化蛋白 1蛋白含量明显减低 (P均 <0 .0 0 1)。以上结果提示 ,氯沙坦可拮抗血管紧张素Ⅱ所致的平滑肌细胞内单核细胞趋化蛋白 1的表达与分泌 ,这可能有助于减轻或防治某些病理状态下血管平滑肌细胞的增殖与迁移。Aim To investigate the role of angiotensin Ⅱ receptor antagonist losartan on the protein and mRNA expression of monnocyte chemoattractant protein 1 (MCP 1) in cultured rabbit aortic smooth muscle cells (SMC). Methods The levels of protein and mRNA expression of MCP 1 in cultured SMC and the contents of MCP 1 in the cultured media of SMC were detected by cytochemistry,situ hybridization and enzyme linked immunosorbent assay. Results 10 -6 ～10 -10 mol/L angiotensin Ⅱ(ATⅡ) increased the levels of protein and mRNA expression of MCP 1 in the cultured rabbit aortic SMC in a dose dependent manner and the co)ntents of MCP 1 in the cultured media of of SMC (P<0.001). Losartan markedly reduced the protein and mRNA expression of MCP 1 in the cultured rabbit aortic SMC and the contents of MCP 1 in the cultured media of of SMC induced by ATⅡ. Conclusions Losartan may antagonist ATⅡ induced protein and mRNA expression and secretion of MCP 1 in the cultured rabbit aortic SMC,which might play an important role in preventing the early atherosclerosis and the restenosis after angioplasty.

关 键 词：血管紧张素Ⅱ 单核细胞趋化蛋白-Ⅰ 受体 血管紧张素Ⅱ 受体拮抗剂 氯沙坦 血管平滑肌细胞 

分 类 号：R972.6[医药卫生—药品]