异丹叶大黄素对人滑膜细胞白细胞介素-8生成及mRNA表达的影响  

INHIBITORY EFFECTS OF ISORHAPOTIGENIN ON IL-8 PRODUCTION AND mRNA EXPRESSION INDUCED WITH TNFα IN NORMAL HUMAN SYNOVIAL CELLS

作　　者：侯琦[1] 李良成[1] 郭颖[1] 程桂芳[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2001年第6期407-410,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 ( 39770 911)

摘　　要：目的　研究异丹叶大黄素 (isorhapotigenin ,Iso)对肿瘤坏死因子α(TNF α)诱导的人滑膜细胞 (humansynovialcell,HSC)白细胞介素 8(IL 8)生成和mRNA表达的影响。 方法　用RIA方法测定IL 8的含量 ,以RT PCR法测IL 8mRNA。结果　Iso在 1× 10 -6 - 1× 10 -5mol·L-1浓度范围内对TNF α诱导的人滑膜细胞IL 8生成有抑制作用 ,并抑制TNF α诱导的HSCIL 8mRNA表达。结论　Iso抑制TNF α诱导的HSCIL 8生成 ,可能与影响ILAIM To study the effects of isorhapotigenin (Iso) on interleukin 8 (IL 8) production and mRNA expression in normal human synovial cells (HSC) induced with TNFα. METHODS IL 8 were assayed with RIA method. The mRNA expression of IL 8 was detected by RT PCR method. RESULTS It was shown that TNFα at concentrations of 0 05 to 0 5 U·mL -1 for 24 h significantly increased IL 8 production. The expression of IL 8 mRNA was also promoted by TNFα (0 25 U·mL -1 ) for 6 h. Iso at the concentrations of 1×10 -6 mol·L -1 to 1×10 -5 mol·L -1 showed inhibitory effects on IL 8 production induced with TNFα (0 25 U·mL -1 ). The further study indicated that Iso at the concentrations of 1×10 -6 mol·L -1 to 1×10 -5 mol·L -1 inhibited IL 8 mRNA expression in HSC induced with TNFα (0 25 U·mL -1 ). CONCLUSION TNFα promoted IL 8 production and mRNA expression in HSC. Iso inhibited IL 8 production and mRNA expression induced by TNFα (0 25 U·mL -1 ). This might be one of the anti inflammatory mechanisms of Iso.

关 键 词：异丹叶大黄素 白细胞介素-8 放免测定法 反转录聚合酶链式反应 人滑膜细胞 MRNA 

分 类 号：R967[医药卫生—药理学]