哮喘大鼠血清刺激下内皮细胞表面粘附分子ICAM-1的表达  

Expression Property of Adhesive Molecule ICAM-1 on Vitro cultured Pulmonary Microvascular Endothelial Cells Under the Stimuli of Asthmatic Serum

作　　者：盛佳[1] 曾衍钧[1] 庄逢源[2] 

机构地区：[1]北京工业大学生物力学教研室,北京100022 [2]中日友好临床医学研究所血流变与微循环研究室,北京100029

出　　处：《生物医学工程学杂志》2001年第2期192-194,205,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (39470 195 );北京自然科学基金资助项目 (3982 0 0 5 )

摘　　要：近十五年人们才发现炎症是哮喘发病的病理基础。它以白细胞浸润为主要特征 ,过程由白细胞与内皮细胞表面粘附分子介导 ,ICAM- 1就是其中之一。我们在前期活体动物实验中证实了哮喘动物肺组织中内皮细胞 -白细胞粘附现象显著 ,并且肺组织 ICAM- 1高表达。这有可能是发病过程中 ICAM- 1大量表达于内皮细胞表面 ,导致内皮细胞 -白细胞粘附增加的结果。本实验采用肺组织机械分离法体外培养大鼠肺内皮细胞 ,将哮喘病理血清与内皮细胞共同孵育 ,用于细胞粘附的体外研究。借助间接免疫荧光标记技术及流式细胞分离法 ,我们首先研究了受哮喘病理血清刺激后肺微血管内皮细胞表面 ICAM- 1表达的情况。结果发现 :血清孵育的内皮细胞 ICAM- 1表达比用 DMEM培养基培养的内皮细胞表达量高 ;哮喘血清刺激 4h后 ICAM- 1表达量达到峰值 ,长于 4h则很快降低 ;It has been recognized only in the past 15 years that the major pathological mechanism of asthma is inflammation, which has a hallmark of focal leukocyte extravasation. The different recruitment behaviors of inflammatory cells depend on the expression of specific adhesive molecules on both leukocytes and endothelial cells (EC). Intercellular cellular adhesion molecule-1 (ICAM-1) plays a major role. In the previous detailed in-vivo study of asthmatic models, we have proved the significant pathological increase of both the leukocyte-endothelium adhesion phenomenon and the ICAM-1 expression in the tissues of asthmatic lung. It may be due to an ICAM-1 accumulation on the microvascular endothelial cells and thus an enhancement of adhesion force during the course of disease. In this study, endothelial cells were obtained from rats by mechanical isolation of lung tissues and in vitro culture on glass. Confluent endothelial cells were incubated with serum collected from rat blood of normal and asthmatic models a nd were used for in vitro study of endothelial cell adhesion. The effect of pathological serum stimulation was examined on the expression of ICAM-1 of the pulmonary microvascular endothelial cells (PMEC). The expression of ICAM-1 on PMEC was measured by indirect immuno-fluorescence with flow cytometry. We found that the surface expression of ICAM-1 was obviously increased on serum-incubated EC as compared with that on the culture solution-incubated one, and that asthmatic serum increased the expression of ICAM-1 on EC to a peak in 4 hours and then decreased it rapidly, the expression level remained the same in the whole course when EC was treated with culture solution or normal serum.

关 键 词：哮喘 内皮细胞 表面粘附分子 ICAM-1 

分 类 号：R562.25[医药卫生—呼吸系统]