砷化物对HL-60细胞生长及基因组DNA甲基化的影响  

Effects of arsenicals on growth and genomic DNA methylation in HL 60 cells

作　　者：卢光明[1] 崔旭[1] 黄福南[1] 赵军平[1] 戚其平[2] 姚孝远[2] 丛涛[1] 

机构地区：[1]解放军总医院基础所,北京100853 [2]中国预防医学科学院环监所,北京100021

出　　处：《中国地方病学杂志》2001年第3期161-163,共3页Chinese Jouranl of Endemiology

基　　金：1999-2 0 0 1年国家自然科学基金资助项目 (3890 0 12 3)

摘　　要：目的　研究 Na As O2 和 Na3As O4对 HL- 6 0细胞生长及基因组 DNA甲基化的影响。方法　采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组 DNA甲基化水平。结果　 1两种无机砷化物对 HL- 6 0细胞生长具有抑制作用 ,特别是高浓度 Na As O2 (2、10 μmol/ L)和 Na3As O4(10 0、30 0 μmol/ L)可明显地抑制 HL- 6 0细胞的生长 (P <0 .0 1)。2随着砷化物浓度的增加 ,Msp 酶解基因组 DNA片段的分子量变化不大 ,但 Hpa 酶解片段的分子量逐渐变大。结论　提示基因组 DNA中 CCGG核苷酸内侧 C的甲基化总的水平有上升趋势。Objective To study the effects of arsenicals on growth and genomic DNA methylation of HL 60 cells.Methods Cell culture and methylation sensitive restriction endonuclease digestion were used.Results ①Arsenicals caused inhibition obviously on HL 60 cells growth at the test concentrations, particularly in the groups of 2μmol/L, 10μmol/L sodium arsenite,and the groups of 100 μmol/L, 300 μmol/L sodium arsenate.②The length of HpaⅡrestriction fragments were substantially increased concurrently with the increasing of arsenic doses for treating the HL 60 cells, and no change was observed for MspI cutting bands. Conclusions Arsenicals can inhibit the growth of HL 60 cells, and cause hypermethylation in internal cytosine-methylation in the C mCGG sequence of the total genomic DNA.

关 键 词：砷化物 细胞生长 脱氧核糖核酸甲基化 DNA 毒性 HL-60细胞 致癌物 

分 类 号：R992[医药卫生—毒理学]