番茄离体培养的形态发生  

Morphogenesis of Lycopersion esculentum Mill Cultured in Vitro

作　　者：陈火英[1] 张建华[2] 俞俊棠[1] 钟建江[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海交通大学植物科学系,上海201101

出　　处：《华东理工大学学报（自然科学版）》2001年第2期139-142,共4页Journal of East China University of Science and Technology

基　　金：上海市曙光资助项目 !(98SG47)

摘　　要：对番茄下胚轴、子叶、叶片、叶柄不同类型外植体离体培养中有关细胞启动、分裂、分化以及器官发生作了细胞组织学观察 ,结果表明 :番茄不同类型外植体在同样的培养条件下 ,愈伤组织生长表现明显差异 :下胚轴、子叶诱导产生愈伤组织时 ,细胞启动早 ,生长快 ,分裂方式基本为无丝分裂 ;下胚轴诱导愈伤组织形成时 ,细胞不规则的无丝分裂少于子叶 ,故下胚轴离体培养得到正常芽的比例高于子叶 ;番茄离体培养中不定芽通常发生在愈伤组织的周边区 ,也可起源于维管组织结节周围 ,形成层状细胞。Hypocotyl, cotyledon, leaf blade and leaf petiole explants of tomato were cultured on MS medium supplemented with 2.0mg/L BA and 0.2mg/L IAA for induction of callus and bud formation. Histological observations on callus and bud formation were carried out. The following results were obtained: there were significant difference in callus induction and growth among above mentioned 3 kinds of explants, calli from hypocotyl and cotyledon explants were induced earlier than others, and grew faster than others. Buds differentiated from calli were adventitious buds. Adventitious buds were generally formed on the peripheral parts of the calli, also originated from the cambium like cells surrounded by the vascular nodules. Adventitious roots originated from the pericycle. The caryological observation on callus paraffin section showed that, in the course of callus formation from cotyledon there were more kinds of nuclear division modes than hypocotyl calli, but all these were of amitosis.

关 键 词：番茄 外植体 离体培养 形态发生 组织学观察 

分 类 号：S641.2[农业科学—蔬菜学] Q943.1[农业科学—园艺学]