双缩水甘油醚活化法制备免疫吸附剂及其对乙型肝炎表面抗原的吸附  

THE PREPARATION OF IMMUNOADSORBENT BY ACTIVATION OF 1,4-BUTANEDIOL DIGLYCIDYL ETHER AND THE ADSORPTION FOR HEPATITIS B SURFACE ANTIGEN

作　　者：李湛勇[1] 史林启[1] 何炳林[1] 梁树人[2] 

机构地区：[1]吸附分离功能高分子材料国家重点实验室 [2]天津市传染病院,天津300192

出　　处：《离子交换与吸附》2001年第3期236-241,共6页Ion Exchange and Adsorption

基　　金：国家自然科学基金(59503005)

摘　　要：采用 1,4-二羟基正丁烷双缩水甘油醚活化交联壳聚糖树脂，偶联抗-乙型肝炎表面抗 原单克隆抗体，制备树脂/抗体免疫吸附剂。对含乙型肝炎表面抗原的患者阳性血清的吸附 试验结果表明，吸附率可达44%，能使阳性血清转为阴性。The crosslinked chitosan resins were activated by 1,4-butanediol digly cidyl ether. The effect factors of activation were studied in detail and the rea ction conditions were optimized. Then the anti-HBsAg monoclonal antibody were co upled to the activated carrier to obtain the immunoadsorbent. The adsorption pro perties of the immunoadsorbent were studied in serum containing HBsAg and the ad sorption efficiency reached 44%.

关 键 词：免疫地剂 1 4-二羟基正丁烷双缩水甘油醚 交联壳聚糖树脂 乙型肝炎 表面抗 治疗 血液净化 

分 类 号：R512.62[医药卫生—内科学] R457[医药卫生—临床医学]