环氧活化法制备免疫吸附剂及其对乙型肝炎表面抗原的吸附  

THE PREPARATION OF IMMUNOADSORBENT WITH EPOXY-GROUP AND THE ADSORPTION FOR HEPAT ITIS B SURFACE ANTIGEN

作　　者：李湛勇[1] 史林启[1] 何炳林[1] 梁树人[2] 

机构地区：[1]吸附分离功能高分子材料国家重点实验室 [2]天津市传染病院,天津300192

出　　处：《离子交换与吸附》2001年第3期242-247,共6页Ion Exchange and Adsorption

基　　金：国家自然科学基金(59503005)

摘　　要：分别采用环氧氯丙烷和1,4-二羟基正丁烷双缩水甘油醚活化交联壳聚糖树脂，经偶联 抗-乙型肝炎表面抗原(HBsAg)单克隆抗体，制备树脂/抗体免疫吸附剂。含HBsAg的患者阳性 血清的吸附实验结果表明，对HBsAg的吸附率可达44%，能使阳性血清转为阴性。通过对不同 活化试剂制备的免疫吸附剂的活化过程和吸附性能的研究发现，活化试剂中的“手臂”结构 有利于保持偶联抗体的天然构象，使之具有较高的活性。The crosslinked chitosan resins were activated by epichlorohydrin and 1,4-butanediol diglycidyl ether, respectively. The effect factors of activation were studied in detail and the reaction conditions were optimized. Then the anti -HBsAg monoclonal antibodies were bound to the activated carrier to obtain the i mmunoadsorbent. The adsorption properties of the immunoadsorbent were studied in serum containing HBsAg and the adsorption efficiency reached 44%. With the furt her studies on the activation and the adsorption, a conclusion was draw that the activation reagent with longer spacer was favor to keep the normal configuratio n of the antibodies bound to the adsorbent carriers so that the immunoadsorbent was provided with higher bioactivity to react with HBsAg.

关 键 词：免疫吸附剂 交联壳聚糖树脂 环氧氯丙烷 1 4-二羟基正丁烷双缩水甘油醚 2型肝炎表面抗原 治疗 血液净化 

分 类 号：R512.62[医药卫生—内科学] R457[医药卫生—临床医学]