聚酰胺-胺型树枝状化合物与细胞色素C的结合作用  

THE CONJUGATION OF POLY(AMINOAMINE) DENDRIMERS (PAMAM) AND CYTOCHROME C

作　　者：陈丽蓉[1] 钟竑 贾欣茹[2] 秦洋[2] 廖清[1] 李明谦[2] 危岩 

机构地区：[1]北京大学技术物理系,北京100080 [2]北京大学化学与分子工程学院,北京100871 [3]美国Drexel大学化学系,费城pa19104

出　　处：《高分子学报》2001年第4期553-556,共4页Acta Polymerica Sinica

基　　金：国家自然科学基金资助课题 (基金号 5 9973 0 0 1)

摘　　要：The methyl ester terminated G1 5,2 5 and 3 5 poly(amidoamine) dendrimers (PAMAM) are hydrolyzed by using NaOH to obtain external carboxylate groups with them (PAMAMC).These dendrimers show interaction with cytochrome C in some extent.For example,the reduction rate of Fe Ⅲ cytochrome C to Fe Ⅱ cytochrome C by ascorbic acid was clearly slowed down because of the interaction between cytochrome C and PAMAM dendrimers.The reaction happened only betweem Fe Ⅲ cytochrome C and ascorbic acid,it showed a pseudo first order constant of 3 48 min -1 .When PAMAMC (G2 5 or 3 5) dendrimers were added in the reaction system,the pseudo first order constant dropped to 1 40 or 1 32 ?min -1 .The association constant of 1:1 cytochrome C G2 5C is 2 4×10 4 and the association constant of 1:1 cytochrome C G3.5C is 2 6×10 4.The methyl-ester-terminated G1.5, 2.5 and 3.5 poly (amidoamine) dendrimers (PAMAM) are hydrolyzed by using NaOH to obtain external carboxylate groups with them (PAMAMC). These dendrimers show interaction with cytochrome C in some extent. For example, the reduction rate of Fe-III-cytochrome C to Fe-II-cytochrome, C by ascorbic acid was clearly slowed down because of the interaction between cytochrome C and PAMAM dendrimers. The reaction happened only between Fe-III-cytochrome C and ascorbic acid, it showed a pseudo-first order constant of 3.48 min(-1). When PAMAMC (G2.5 or 3.5) dendrimers were added in the reaction system, the pseudo-first order constant dropped to 1.40 or 1.32 min(-1) The association constant of 1: 1 cytochrome C-G2.5C is 2.4 x 10(4) and the association constant of 1: 1 cytochrome C-G3.5C is 2.6 x 10(4).

关 键 词：树状化合物 蛋白质 细胞色素C 分子识别 聚酰胺 相互作用 构象 

分 类 号：O631[理学—高分子化学]