SDS-Schiff碱铜(Ⅱ)配合物模拟酶催化光度法研究  

Studies on the Catalytic Spectrophotometry Using SDS-Schiff Cu(Ⅱ) Complex as Mimetic Enzyme

作　　者：唐波[1] 刘阳[1] 唐晓玲[2] 

机构地区：[1]山东师范大学化学系,山东师范大学生物系济南250014 [2]山东师范大学生物系,济南250014

出　　处：《高等学校化学学报》2001年第6期919-921,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :2 9975 0 16)资助

摘　　要：A new Schiff base, 2 hydroxy 1 napthaldehyde 2 amino thiazole(HNATS) and its Cu(Ⅱ) complex was synthesized. The complex[Cu(Ⅱ) (HNATS) 2] could exhibit an efficient peroxitase like catalytic activity, and catalyze the coupling reaction of 2,4 dichlorophenol and 4 amino antipyrine with hydrogen peroxide. The effect of SDS on the catalytic activity of mimetic enzyme was studied. Based on the reaction, the determination of O ·- 2 by this new spectrophotometric method was developed. The proposed method was used to determine SOD activity of garlic, scallion and onion with satisfactory results.A new Schiff base, 2 hydroxy 1 napthaldehyde 2 amino thiazole(HNATS) and its Cu(Ⅱ) complex was synthesized. The complex[Cu(Ⅱ) (HNATS) 2] could exhibit an efficient peroxitase like catalytic activity, and catalyze the coupling reaction of 2,4 dichlorophenol and 4 amino antipyrine with hydrogen peroxide. The effect of SDS on the catalytic activity of mimetic enzyme was studied. Based on the reaction, the determination of O ·- 2 by this new spectrophotometric method was developed. The proposed method was used to determine SOD activity of garlic, scallion and onion with satisfactory results.

关 键 词：表面活性剂 模拟酶 催化光度法 超氧阴离子自由基 超氧化物歧化酶 测定 SDS 西佛碱 铜(Ⅱ)配合物 

分 类 号：Q554.9[生物学—生物化学] O657.32[理学—分析化学]