丝组二肽对牛血清白蛋白的切割作用研究  

Studies on the Cleavage of Bovine Serum Albumin by Ser-His

作　　者：陈晶[1] 万荣[1] 刘海[1] 蒋宇扬[1] 赵玉芬[1] 

机构地区：[1]清华大学生命科学与工程研究院,化学系生命有机磷化学教育部重点实验室,北京100084

出　　处：《高等学校化学学报》2001年第8期1349-1351,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学重点项目基金 (批准号 :2 96 32 0 0 4);访问学者基金 (批准号 :教技司 [1999] 15 3号 )

摘　　要：It has been found that Ser His could cleave bovine serum albumin(BSA), with the most suitable pH around 6.0. The kinds of buffers have different influences on the cleavage activity. The phosphate buffer and BR buffer are the most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and carboxyl group of Ser His increase the cleavage activity.It has been found that Ser His could cleave bovine serum albumin(BSA), with the most suitable pH around 6.0. The kinds of buffers have different influences on the cleavage activity. The phosphate buffer and BR buffer are the most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and carboxyl group of Ser His increase the cleavage activity.

关 键 词：丝组二肽 牛血清白蛋白 切割 化学切割试剂 蛋白酶 模拟 

分 类 号：Q512.1[生物学—生物化学] Q556.9