血红蛋白与NO分子间相互作用的电化学表征  

Electrochemical Characterization on the Interaction Between Hemoglobin and NO Molecule

作　　者：李清文[1] 高宏[1] 黄昊[1] 王义明[1] 冯军[1] 罗国安[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《高等学校化学学报》2001年第8期1373-1375,共3页Chemical Journal of Chinese Universities

基　　金：国家九七三项目 (批准号 :G19990 5 440 4);国家自然科学基金重点项目 (批准号 :2 0 0 75 0 15 )

摘　　要：Nanocrystalline TiO 2 particles film has been considered as an eminent matrix for the immobilization of biomolecules due to its high biocompatiability, high protein loading and special binding ability. The direct electron transfer process of hemoglobin was achieved at nanosized TiO 2 film electrode, in which a well defined redox peak of hemoglobin could be observed. However, this peak decreased with the presence of NO, indicating the electrochemical activity of hemoglobin was inhibited by the interation between hemoglobin and NO. While the further evidence revealed that such activity could be recovered after releasing NO from hemoglobin with electrochemical oxidation method.Nanocrystalline TiO 2 particles film has been considered as an eminent matrix for the immobilization of biomolecules due to its high biocompatiability, high protein loading and special binding ability. The direct electron transfer process of hemoglobin was achieved at nanosized TiO 2 film electrode, in which a well defined redox peak of hemoglobin could be observed. However, this peak decreased with the presence of NO, indicating the electrochemical activity of hemoglobin was inhibited by the interation between hemoglobin and NO. While the further evidence revealed that such activity could be recovered after releasing NO from hemoglobin with electrochemical oxidation method.

关 键 词：血红蛋白 分子间相互作用 电化学方法 一氧化氮 纳米氧化钛涂层电极 

分 类 号：Q51[生物学—生物化学] O646.5[理学—物理化学]